Thakur D C, Saumya Kumar Udit, Singh Vipendra Kumar, Giri Rajanish
School of Biosciences and Bioengineering, Indian Institute of Technology Mandi, VPO Kamand, Mandi 175075, Himachal Pradesh, India.
ACS Omega. 2025 Jul 24;10(30):32673-32679. doi: 10.1021/acsomega.4c02809. eCollection 2025 Aug 5.
Signal peptides (SPs) are essential tools for sorting or translocating synthesized proteins. However, except for a few examples, their studies in isolation are quite limited. Here, we asked a question about the aggregation propensity of signal peptides (residues 1-18) of Human Serum Albumin (HSA). Molecular dynamic simulations were used to observe the mechanism through which signal peptides of HSA self-aggregated under physiological conditions. Further, we confirmed our results by combining dye-based assays, atomic force microscopy, and transmission electron microscopy techniques. It was noted that the signal peptide of HSA (regions 1-18) forms typical amyloid-like fibrils.
信号肽（SPs）是用于分选或转运合成蛋白的重要工具。然而，除了少数例子外，对它们的单独研究相当有限。在此，我们提出了一个关于人血清白蛋白（HSA）信号肽（第1-18位残基）聚集倾向的问题。利用分子动力学模拟来观察HSA信号肽在生理条件下自我聚集的机制。此外，我们通过结合基于染料的检测、原子力显微镜和透射电子显微镜技术来证实我们的结果。值得注意的是，HSA的信号肽（第1-18位区域）形成了典型的淀粉样纤维。
