Sad S, Talwar G P, Raghupathy R
National Institute of Immunology, J.N.U. Complex, New Delhi, India.
J Reprod Immunol. 1991 Feb;19(2):197-207. doi: 10.1016/0165-0378(91)90017-k.
A vaccine against the gonadotropin releasing hormone (GnRH) is being developed as an immunological method for the treatment of prostatic enlargement. The work described here was aimed at investigating the influence of the genetic background on immune responses to GnRH conjugated to diphtheria toxoid (DT). Mice of different strains were injected with the conjugate and the antibody levels against GnRH and DT quantitated in order to examine the effect of genetic background on immune responses to the hapten and the carrier. All immunized animals produced antibodies to DT. Anti-GnRH antibodies were generated by all strains of mice except 129. The low anti-GnRH response in the 129 strain mice did not appear to be MHC-linked, as C57BL/6 mice, which bear the same MHC haplotype as 129 mice, were able to generate a strong anti-GnRH response. The non-responsiveness to the hapten (GnRH) in 129 strain mice was overcome by the use of an 'alternate carrier' approach.
一种针对促性腺激素释放激素（GnRH）的疫苗正在研发中，作为治疗前列腺增生的一种免疫方法。本文所述工作旨在研究遗传背景对与白喉类毒素（DT）偶联的GnRH免疫反应的影响。给不同品系的小鼠注射该偶联物，并对针对GnRH和DT的抗体水平进行定量，以检查遗传背景对对半抗原和载体免疫反应的影响。所有免疫动物均产生了针对DT的抗体。除129品系外，所有小鼠品系均产生了抗GnRH抗体。129品系小鼠中抗GnRH反应较低，这似乎与主要组织相容性复合体（MHC）无关，因为与129小鼠具有相同MHC单倍型的C57BL/6小鼠能够产生强烈的抗GnRH反应。通过使用“替代载体”方法克服了129品系小鼠对半抗原（GnRH）的无反应性。
