Israel Oceanographic and Limnological Research, Tel-Shikmona, Haifa 31080, Israel.
Dev Comp Immunol. 2010 Jun;34(6):630-7. doi: 10.1016/j.dci.2010.01.007. Epub 2010 Jan 25.
While reef-building corals portray highly complex and specific allorecognition responses, still, no available synthesis on historecognition at the molecular level exists for this group of organisms. Here, we present the first subtractive library of expressed sequence tags (ESTs) from allogeneic challenged coral (Stylophora pistillata) colonies revealing the differential expression of a wide range of immune-related genes. 1760 unique ESTs were clustered and assembled into 230 contigs and 1530 singlets with 28% that showed homology (E-value < or =0.005) to known database sequences, of which 16% (n=80) homologues were identified as immune-relevant genes, encoding for stress proteins, pattern recognition receptors and complement proteins, proteases, cell adhesion proteins, cytokine related proteins, programmed cell death and proteasome-associated proteins. Transcripts that were subjected to quantitative RT-PCR, further supported the library data. In situ hybridization analyses elucidated specific and enhanced expressions of von Willebrand factor-like transcript during S. pistillata allogeneic rejection. Availability of such genome-wide expression tools may lead to significant advances in the research of coral historecognition and comparative immunology.
虽然造礁珊瑚表现出高度复杂和特定的异体识别反应，但目前还没有关于该类生物体在分子水平上的组织识别的综合研究。在这里，我们展示了第一个来自同种异体挑战珊瑚（石珊瑚属）群体的表达序列标签（EST）的消减文库，揭示了广泛的免疫相关基因的差异表达。1760 个独特的 EST 被聚类并组装成 230 个重叠群和 1530 个单序列，其中 28%（E 值<或=0.005）与已知数据库序列具有同源性，其中 16%（n=80）同源物被鉴定为免疫相关基因，编码应激蛋白、模式识别受体和补体蛋白、蛋白酶、细胞粘附蛋白、细胞因子相关蛋白、程序性细胞死亡和蛋白酶体相关蛋白。定量 RT-PCR 进一步支持了文库数据。原位杂交分析阐明了 von Willebrand 因子样转录本在石珊瑚同种异体排斥中的特异性和增强表达。此类全基因组表达工具的可用性可能会推动珊瑚组织识别和比较免疫学研究的重大进展。
