Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Seizure. 2010 Mar;19(2):84-92. doi: 10.1016/j.seizure.2009.12.003. Epub 2010 Jan 18.
In this study, we investigated the therapeutic potential of bone marrow mononuclear cells (BMCs) in a model of epilepsy induced by pilocarpine in rats. BMCs obtained from green fluorescent protein (GFP) transgenic mice or rats were transplanted intravenously after induction of status epilepticus (SE). Spontaneous recurrent seizures (SRS) were monitored using Racine's seizure severity scale. All of the rats in the saline-treated epileptic control group developed SRS, whereas none of the BMC-treated epileptic animals had seizures in the short term (15 days after transplantation), regardless of the BMC source. Over the long-term chronic phase (120 days after transplantation), only 25% of BMC-treated epileptic animals had seizures, but with a lower frequency and duration compared to the epileptic control group. The density of hippocampal neurons in the brains of animals treated with BMCs was markedly preserved. At hippocampal Schaeffer collateral-CA1 synapses, long-term potentiation was preserved in BMC-transplanted rats compared to epileptic controls. The donor-derived GFP(+) cells were rarely found in the brains of transplanted epileptic rats. In conclusion, treatment with BMCs can prevent the development of chronic seizures, reduce neuronal loss, and influence the reorganization of the hippocampal neuronal network.
在这项研究中，我们研究了骨髓单核细胞（BMCs）在匹罗卡品诱导的大鼠癫痫模型中的治疗潜力。在诱导癫痫持续状态（SE）后，从绿色荧光蛋白（GFP）转基因小鼠或大鼠中获得 BMCs，并通过静脉内移植。使用 Racine 的癫痫严重程度量表监测自发性反复性癫痫发作（SRS）。所有接受生理盐水治疗的癫痫对照组大鼠均出现 SRS，而无论 BMC 来源如何，接受 BMC 治疗的癫痫动物在短期内（移植后 15 天）均无癫痫发作。在长期慢性期（移植后 120 天），只有 25%的 BMC 治疗的癫痫动物出现癫痫发作，但与癫痫对照组相比，其频率和持续时间较低。接受 BMC 治疗的动物的海马神经元密度明显保留。在接受 BMC 移植的大鼠的海马 Schaeffer 侧枝-CA1 突触中，长时程增强作用得到保留，与癫痫对照组相比。在接受移植的癫痫大鼠的大脑中很少发现供体衍生的 GFP(+)细胞。总之，BMCs 的治疗可以预防慢性癫痫发作的发展，减少神经元丢失，并影响海马神经网络的重组。
