Department of Pathology and Laboratory Medicine, Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
J Biol Chem. 2010 Mar 12;285(11):8148-54. doi: 10.1074/jbc.M109.089821. Epub 2010 Jan 15.
Recent studies have uncovered an unexpected relationship between factors that are essential for germline development in Drosophila melanogaster: the arginine protein methyltransferase 5 (dPRMT5/Csul/Dart5) and its cofactor Valois, methylate the Piwi family protein Aub, enabling it to bind Tudor. The RNA helicase Vasa is another essential protein in germline development. Here, we report that mouse (mouse Vasa homolog), Xenopus laevis, and D. melanogaster Vasa proteins contain both symmetrical and asymmetrical dimethylarginines. We find that dPRMT5 is required for the production of sDMAs of Vasa in vivo. Furthermore, we find that the mouse Vasa homolog associates with Tudor domain-containing proteins, Tdrd1 and Tdrd6, as well as the Piwi proteins, Mili and Miwi. Arginine methylation is thus emerging as a conserved and pivotal post-translational modification of proteins that is essential for germline development.
最近的研究揭示了一个出人意料的关系，即果蝇生殖系发育所必需的因素之间的关系：精原蛋白甲基转移酶 5（dPRMT5/Csul/Dart5）及其辅助因子瓦卢瓦，甲基化 Piwi 家族蛋白 Aub，使其能够结合 Tudor。RNA 解旋酶 Vasa 是生殖系发育的另一种必需蛋白。在这里，我们报告说，老鼠（老鼠 Vasa 同源物）、非洲爪蟾和黑腹果蝇 Vasa 蛋白都含有对称和不对称二甲基精氨酸。我们发现 dPRMT5 是体内 Vasa 的 sDMAs 产生所必需的。此外，我们发现小鼠 Vasa 同源物与含有 Tudor 结构域的蛋白 Tdrd1 和 Tdrd6 以及 Piwi 蛋白 Mili 和 Miwi 结合。因此，精氨酸甲基化作为一种保守的、关键的蛋白质翻译后修饰，对生殖系发育至关重要。
