快速而特异的方法评价复杂土壤群落中链霉菌的竞争动态。
Rapid and specific method for evaluating Streptomyces competitive dynamics in complex soil communities.
机构信息
Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, Minnesota 551081, USA.
出版信息
Appl Environ Microbiol. 2010 Mar;76(6):2009-12. doi: 10.1128/AEM.02320-09. Epub 2010 Jan 15.
Abstract
Quantifying target microbial populations in complex communities remains a barrier to studying species interactions in soil environments. Quantitative PCR (qPCR) assays were developed for quantifying pathogenic Streptomyces scabiei and antibiotic-producing Streptomyces lavendulae strains in complex soil communities. This assay will be useful for evaluating the competitive dynamics of streptomycetes in soil.
摘要
量化复杂群落中的目标微生物种群仍然是研究土壤环境中物种相互作用的障碍。开发了定量 PCR（qPCR）测定法来量化复杂土壤群落中的致病疮痂链霉菌和产生抗生素的薰衣草链霉菌菌株。该测定法将有助于评估土壤中链霉菌的竞争动态。
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