利用 15N 和 13C 掺入法检测 2,4,6-三硝基甲苯利用厌氧细菌。
Detection of 2,4,6-trinitrotoluene-utilizing anaerobic bacteria by 15N and 13C incorporation.
机构信息
Department of Environmental Sciences, the State University of New Jersey, New Brunswick, New Jersey 08901, USA.
出版信息
Appl Environ Microbiol. 2010 Mar;76(5):1695-8. doi: 10.1128/AEM.02274-09. Epub 2010 Jan 15.
Abstract
2,4,6-Trinitrotoluene ((15)N or (13)C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [(15)N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [(13)C]TNT cultures and was related to Lysobacter taiwanensis.
摘要
2,4,6-三硝基甲苯（(15)N 或 (13)C 标记）被添加到诺福克港沉积物中，以测试厌氧细菌是否将 TNT 用于生长。稳定同位素探测（SIP）-末端限制性片段长度多态性（TRFLP）在 [(15)N]TNT 培养物中检测到峰（60、163 和 168 bp）。60-bp 峰也存在于 [(13)C]TNT 培养物中，与 Lysobacter taiwanensis 有关。
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