通过DNA稳定同位素探测鉴定产硫化物苯降解菌群中的关键成员
Identification of critical members in a sulfidogenic benzene-degrading consortium by DNA stable isotope probing.
作者信息
Oka A R, Phelps C D, McGuinness L M, Mumford A, Young L Y, Kerkhof L J
机构信息
Department of Environmental Sciences1 and Institute of Marine and Coastal Sciences, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA.
出版信息
Appl Environ Microbiol. 2008 Oct;74(20):6476-80. doi: 10.1128/AEM.01082-08. Epub 2008 Aug 29.
Abstract
Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the (13)C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.
摘要
稳定同位素探测（SIP）被用于识别一个降解苯的硫酸盐还原菌联合体中的活性成员。SIP-末端限制性片段长度多态性分析表明，一个270 bp的峰整合了大部分的（13）C标记，并且是一个与克隆SB-21（GenBank登录号AF029045）序列密切相关的序列。该目标可能是该领域中厌氧苯降解的一个重要生物标志物。
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