F 质粒 TraF 和 TraH 是参与接合的外膜复合物的组成部分。
F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation.
机构信息
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
出版信息
J Bacteriol. 2010 Mar;192(6):1730-4. doi: 10.1128/JB.00726-09. Epub 2010 Jan 15.
Abstract
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH.
摘要
F 质粒 TraF 和 TraH 是 F 菌毛组装和 F 质粒转移所必需的。使用漂浮蔗糖密度梯度，我们发现 TraF 和 TraH（以及 TraU 和 TraW）在外膜中定位，尤其是 TraV，假定的锚。TraH 的突变分析揭示了两个对其功能很重要的结构域，可能与 TrbI 相互作用，而 TrbI 又在稳定 TraH 方面发挥作用。
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