• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

体外重建四嗪酸盐 RK-682 生物合成。

In vitro reconstruction of tetronate RK-682 biosynthesis.

机构信息

Department of Biochemistry, University of Cambridge, Cambridge, UK.

出版信息

Nat Chem Biol. 2010 Feb;6(2):99-101. doi: 10.1038/nchembio.285. Epub 2009 Dec 20.

DOI:10.1038/nchembio.285
PMID:20081823
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2811812/
Abstract

The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccharopolyspora erythraea and reconstitution of the RK-682 pathway using recombinant enzymes, and we show that RkD is the enzyme required for RK-682 formation from acyl carrier protein-bound substrates.

摘要

蛋白磷酸酶抑制剂 RK-682 是一类具有潜在价值的四酮聚酮天然产物之一。由于推测的底物难以接近，因此无法了解四酮环的形成方式。我们报告了在红色糖多孢菌中异源表达 rk 基因，并使用重组酶重新构建了 RK-682 途径，结果表明 RkD 是将酰基辅酶 A 结合的底物转化为 RK-682 所需的酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/785965248281/ukmss-28007-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/06839b2f5bad/ukmss-28007-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/c5aedba13d3c/ukmss-28007-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/077f6fdf6011/ukmss-28007-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/785965248281/ukmss-28007-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/06839b2f5bad/ukmss-28007-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/c5aedba13d3c/ukmss-28007-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/077f6fdf6011/ukmss-28007-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ee/2811812/785965248281/ukmss-28007-f0004.jpg

相似文献

1
In vitro reconstruction of tetronate RK-682 biosynthesis.体外重建四嗪酸盐 RK-682 生物合成。
Nat Chem Biol. 2010 Feb;6(2):99-101. doi: 10.1038/nchembio.285. Epub 2009 Dec 20.
2
Is RK-682 a promiscuous enzyme inhibitor? Synthesis and in vitro evaluation of protein tyrosine phosphatase inhibition of racemic RK-682 and analogues.RK-682是一种非特异性酶抑制剂吗？外消旋RK-682及其类似物对蛋白酪氨酸磷酸酶抑制作用的合成与体外评估。
Eur J Med Chem. 2015 Jun 5;97:42-54. doi: 10.1016/j.ejmech.2015.04.036. Epub 2015 Apr 20.
3
Characterization of protein encoded by spnR from the spinosyn gene cluster of Saccharopolyspora spinosa: mechanistic implications for forosamine biosynthesis.来自多刺糖多孢菌的多杀菌素基因簇的spnR编码蛋白的特性：福乐糖胺生物合成的机制意义
J Am Chem Soc. 2005 Jun 1;127(21):7692-3. doi: 10.1021/ja042702k.
4
Design and synthesis of a dimeric derivative of RK-682 with increased inhibitory activity against VHR, a dual-specificity ERK phosphatase: implications for the molecular mechanism of the inhibition.RK-682二聚体衍生物的设计与合成，该衍生物对双特异性ERK磷酸酶VHR的抑制活性增强：对抑制分子机制的启示
Chem Biol. 2001 Dec;8(12):1209-20. doi: 10.1016/s1074-5521(01)00089-8.
5
A new member of the bacterial ribonuclease inhibitor family from Saccharopolyspora erythraea.来自糖多孢红霉菌的细菌核糖核酸酶抑制剂家族的一个新成员。
FEBS Lett. 2004 Jan 16;557(1-3):164-8. doi: 10.1016/s0014-5793(03)01468-6.
6
Polyketide Starter and Extender Units Serve as Regulatory Ligands to Coordinate the Biosynthesis of Antibiotics in Actinomycetes.聚酮合酶起始单元和延伸单元作为调节配体，协调放线菌中抗生素的生物合成。
mBio. 2021 Oct 26;12(5):e0229821. doi: 10.1128/mBio.02298-21. Epub 2021 Sep 28.
7
Dissecting and engineering of the TetR family regulator SACE_7301 for enhanced erythromycin production in Saccharopolyspora erythraea.对四环素阻遏蛋白家族调控因子SACE_7301进行剖析与工程改造以提高红霉糖多孢菌中红霉素的产量。
Microb Cell Fact. 2014 Nov 13;13:158. doi: 10.1186/s12934-014-0158-4.
8
SACE_3986, a TetR family transcriptional regulator, negatively controls erythromycin biosynthesis in Saccharopolyspora erythraea.SACE_3986是一种四环素阻遏蛋白家族转录调节因子，它对糖多孢红霉菌中红霉素的生物合成起负调控作用。
J Ind Microbiol Biotechnol. 2014 Jul;41(7):1159-67. doi: 10.1007/s10295-014-1449-9. Epub 2014 May 3.
9
Asymmetric synthesis of a 3-acyltetronic acid derivative, RK-682, and formation of its calcium salt during silica gel column chromatography.3-酰基四氢呋喃酸衍生物RK-682的不对称合成及其在硅胶柱色谱过程中钙盐的形成。
Chem Pharm Bull (Tokyo). 2001 Feb;49(2):206-12. doi: 10.1248/cpb.49.206.
10
The loading domain of the erythromycin polyketide synthase is not essential for erythromycin biosynthesis in Saccharopolyspora erythraea.在糖多孢红霉菌中，红霉素聚酮合酶的负载结构域对红霉素生物合成并非必不可少。
Microbiology (Reading). 1998 Feb;144 ( Pt 2):543-553. doi: 10.1099/00221287-144-2-543.

引用本文的文献

1
Thiotemplated Polyketide Chain Fusion and Reductive Cyclization Build the Reactive Butenolide Core of Malleicyprol.硫模板聚酮链融合与还原环化构建了马勒西环醇的活性丁烯内酯核心。
Angew Chem Int Ed Engl. 2025 Jul 28;64(31):e202504485. doi: 10.1002/anie.202504485. Epub 2025 Jun 9.
2
A metabologenomics strategy for rapid discovery of polyketides derived from modular polyketide synthases.一种用于快速发现源自模块化聚酮合酶的聚酮化合物的代谢物基因组学策略。
Chem Sci. 2024 Nov 4;16(4):1696-1706. doi: 10.1039/d4sc04174g. eCollection 2025 Jan 22.
3
Dissection of 3D chromosome organization in A3(2) leads to biosynthetic gene cluster overexpression.

本文引用的文献

1
Acyltransferase mediated polyketide release from a fungal megasynthase.酰基转移酶介导的真菌聚酮合酶释放聚酮化合物。
J Am Chem Soc. 2009 Jun 24;131(24):8388-9. doi: 10.1021/ja903203g.
2
New glycosylated derivatives of versipelostatin, the GRP78/Bip molecular chaperone down-regulator, from Streptomyces versipellis 4083-SVS6.来自杂色链霉菌4083 - SVS6的维西泊他汀新糖基化衍生物，GRP78/Bip分子伴侣下调剂。
Org Biomol Chem. 2009 Apr 7;7(7):1454-60. doi: 10.1039/b817312e. Epub 2009 Feb 26.
3
Cloning and characterization of the tetrocarcin A gene cluster from Micromonospora chalcea NRRL 11289 reveals a highly conserved strategy for tetronate biosynthesis in spirotetronate antibiotics.
A3(2) 中三维染色体组织的剖析导致生物合成基因簇的过度表达。
Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2222045120. doi: 10.1073/pnas.2222045120. Epub 2023 Mar 6.
4
Deciphering Chemical Mediators Regulating Specialized Metabolism in a Symbiotic Cyanobacterium.解析调控共生蓝藻特化代谢的化学介质。
Angew Chem Int Ed Engl. 2022 Jun 27;61(26):e202204545. doi: 10.1002/anie.202204545. Epub 2022 May 9.
5
Enzyme alchemy: cell-free synthetic biochemistry for natural products.酶炼金术：用于天然产物的无细胞合成生物化学
Emerg Top Life Sci. 2019 Nov 11;3(5):529-535. doi: 10.1042/ETLS20190083.
6
The biosynthetic pathway to tetromadurin (SF2487/A80577), a polyether tetronate antibiotic.四氢嘧啶（SF2487/A80577）的生物合成途径，一种聚醚四烯抗生素。
PLoS One. 2020 Sep 14;15(9):e0239054. doi: 10.1371/journal.pone.0239054. eCollection 2020.
7
Recent Advances in the Biosynthesis of Carbazoles Produced by Actinomycetes.放线菌产生的咔唑类化合物生物合成的最新进展。
Biomolecules. 2020 Aug 5;10(8):1147. doi: 10.3390/biom10081147.
8
Discovery of Three 22-Membered Macrolides by Deciphering the Streamlined Genome of Mangrove-Derived sp. HM190.通过解析源自红树林的sp. HM190的简化基因组发现三种22元大环内酯类化合物。
Front Microbiol. 2020 Jun 26;11:1464. doi: 10.3389/fmicb.2020.01464. eCollection 2020.
9
Insect-Associated Bacteria Assemble the Antifungal Butenolide Gladiofungin by Non-Canonical Polyketide Chain Termination.昆虫相关细菌通过非典型聚酮链终止组装抗真菌丁烯内酯类化合物灰黄霉素。
Angew Chem Int Ed Engl. 2020 Dec 14;59(51):23122-23126. doi: 10.1002/anie.202005711. Epub 2020 Oct 15.
10
Biosynthesis of Pseudomonas-Derived Butenolides.假单胞菌衍生丁烯内酯的生物合成。
Angew Chem Int Ed Engl. 2020 Mar 27;59(14):5607-5610. doi: 10.1002/anie.201914154. Epub 2020 Jan 29.
来自淡紫小单孢菌NRRL 11289的四抗菌素A基因簇的克隆与特性分析揭示了螺旋四内酯类抗生素中四内酯生物合成的高度保守策略。
J Bacteriol. 2008 Sep;190(17):6014-25. doi: 10.1128/JB.00533-08. Epub 2008 Jun 27.
4
Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic.四环素基因簇分析：对聚醚四内酯抗生素生物合成的见解。
Chembiochem. 2008 May 5;9(7):1136-45. doi: 10.1002/cbic.200700715.
5
Glyceryl-S-acyl carrier protein as an intermediate in the biosynthesis of tetronate antibiotics.甘油 - S - 酰基载体蛋白作为四羟基酸抗生素生物合成中的一种中间体。
Chembiochem. 2008 Jan 4;9(1):150-6. doi: 10.1002/cbic.200700492.
6
Elucidation of the kijanimicin gene cluster: insights into the biosynthesis of spirotetronate antibiotics and nitrosugars.奇加尼霉素基因簇的解析：对螺环四醇酸抗生素和亚硝基糖生物合成的见解。
J Am Chem Soc. 2007 Nov 28;129(47):14670-83. doi: 10.1021/ja0744854. Epub 2007 Nov 7.
7
Terrequinone A biosynthesis through L-tryptophan oxidation, dimerization and bisprenylation.通过L-色氨酸氧化、二聚化和双异戊二烯基化生物合成特瑞醌A。
Nat Chem Biol. 2007 Sep;3(9):584-92. doi: 10.1038/nchembio.2007.20. Epub 2007 Aug 12.
8
Enzymatic total synthesis of enterocin polyketides.肠球菌聚酮化合物的酶促全合成。
Nat Chem Biol. 2007 Sep;3(9):557-8. doi: 10.1038/nchembio.2007.22. Epub 2007 Aug 12.
9
Structure-based dissociation of a type I polyketide synthase module.基于结构的I型聚酮合酶模块解离
Chem Biol. 2007 Jul;14(7):784-92. doi: 10.1016/j.chembiol.2007.05.015.
10
High-throughput, microarray-based synthesis of natural product analogues via in vitro metabolic pathway construction.通过体外代谢途径构建，基于微阵列的天然产物类似物高通量合成。
ACS Chem Biol. 2007 Jun 15;2(6):419-25. doi: 10.1021/cb700033s. Epub 2007 May 25.