放线菌产生的咔唑类化合物生物合成的最新进展。
Recent Advances in the Biosynthesis of Carbazoles Produced by Actinomycetes.
机构信息
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
出版信息
Biomolecules. 2020 Aug 5;10(8):1147. doi: 10.3390/biom10081147.
Structurally diverse carbazole alkaloids are valuable due to their pharmaceutical properties and have been isolated from nature. Experimental knowledge on carbazole biosynthesis is limited. The latest development of in silico analysis of the biosynthetic gene clusters for bacterial carbazoles has allowed studies on the biosynthesis of a carbazole skeleton, which was established by sequential enzyme-coupling reactions associated with an unprecedented carbazole synthase, a thiamine-dependent enzyme, and a ketosynthase-like enzyme. This review describes the carbazole biosynthetic mechanism, which includes a key step in enzymatic formation of a tricyclic carbazole skeleton, followed by modifications such as prenylation and hydroxylation in the skeleton.
结构多样的咔唑生物碱由于其药用性质而具有价值，并已从自然界中分离出来。关于咔唑生物合成的实验知识有限。对细菌咔唑生物合成基因簇进行计算机分析的最新进展，使得对咔唑骨架生物合成的研究成为可能，该骨架是通过与前所未有的咔唑合酶、依赖硫胺素的酶和酮合酶样酶相关的顺序酶偶联反应建立的。本综述描述了咔唑生物合成机制，其中包括酶促形成三环咔唑骨架的关键步骤，以及骨架中的 prenylation 和 hydroxylation 等修饰。
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