Hatta K, Kimmel C B, Ho R K, Walker C
Institute of Neuroscience, University of Oregon, Eugene 97403.
Nature. 1991 Mar 28;350(6316):339-41. doi: 10.1038/350339a0.
The floor plate is a set of epithelial cells present in the ventral midline of the neural tube in vertebrates that seems to have an important role in the developmental patterning of central nervous system fibre pathways, and arrangements of specific neurons. The floor plate arises from dorsal ectodermal cells closely associated with the mesoderm that forms notochord, and it may depend on interactions from the notochord for its specification. To learn the nature of these interactions we have analysed mutations in zebrafish (Brachydanio rerio). We report here that in wild-type embryos the floor plate develops as a simply organized single cell row, but that its development fails in embryos bearing the newly discovered zygotic lethal 'cyclops' mutation, cyc-1(b16). Mosaic analysis establishes that cyc-1 blocks floor plate development autonomously and reveals the presence of homeogenetic induction between floor plate cells.
底板是脊椎动物神经管腹侧中线处的一组上皮细胞，它似乎在中枢神经系统纤维通路的发育模式以及特定神经元的排列中发挥着重要作用。底板起源于与形成脊索的中胚层紧密相连的背侧外胚层细胞，其特化可能依赖于来自脊索的相互作用。为了了解这些相互作用的本质，我们分析了斑马鱼（短盖巨脂鲤）的突变。我们在此报告，在野生型胚胎中，底板发育为简单组织的单细胞排，但在携带新发现的合子致死“独眼”突变cyc-1(b16)的胚胎中，其发育失败。镶嵌分析表明，cyc-1自主阻断底板发育，并揭示了底板细胞之间存在同源诱导。
