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NBD-Cl 对 F1FO ATP 合酶和 A1AO ATP 合酶主要亚基α和 B 的核苷酸结合的影响。

The effect of NBD-Cl in nucleotide-binding of the major subunit alpha and B of the motor proteins F1FO ATP synthase and A1AO ATP synthase.

机构信息

School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Republic of Singapore.

出版信息

J Bioenerg Biomembr. 2010 Feb;42(1):1-10. doi: 10.1007/s10863-009-9266-y. Epub 2010 Jan 16.

DOI:10.1007/s10863-009-9266-y
PMID:20082212
Abstract

Subunit alpha of the Escherichia coli F(1)F(O) ATP synthase has been produced, and its low-resolution structure has been determined. The monodispersity of alpha allowed the studies of nucleotide-binding and inhibitory effect of 4-Chloro-7-nitrobenzofurazan (NBD-Cl) to ATP/ADP-binding. Binding constants (K ( d )) of 1.6 microM of bound MgATP-ATTO-647N and 2.9 microM of MgADP-ATTO-647N have been determined from fluorescence correlation spectroscopy data. A concentration of 51 microM and 55 microM of NBD-Cl dropped the MgATP-ATTO-647N and MgADP-ATTO-647N binding capacity to 50% (IC(50)), respectively. In contrast, no effect was observed in the presence of N,N'-dicyclohexylcarbodiimide. As subunit alpha is the homologue of subunit B of the A(1)A(O) ATP synthase, the interaction of NBD-Cl with B of the A-ATP synthase from Methanosarcina mazei Gö1 has also been shown. The data reveal a reduction of nucleotide-binding of B due to NBD-Cl, resulting in IC(50) values of 41 microM and 42 microM for MgATP-ATTO-647N and MgADP-ATTO-647N, respectively.

摘要

大肠杆菌 F(1)F(O) ATP 合酶的亚基α已被制备，并已确定其低分辨率结构。α的单分散性允许对核苷酸结合和 4-氯-7-硝基苯并呋咱（NBD-Cl）对 ATP/ADP 结合的抑制作用进行研究。荧光相关光谱数据确定了结合的 MgATP-ATTO-647N 的结合常数（K（d））为 1.6 μM，MgADP-ATTO-647N 的结合常数为 2.9 μM。NBD-Cl 的浓度为 51 μM 和 55 μM，将 MgATP-ATTO-647N 和 MgADP-ATTO-647N 的结合能力分别降至 50%（IC（50））。相比之下，在存在 N，N'-二环己基碳二亚胺的情况下，没有观察到任何影响。由于亚基α是 A（1）A（O）ATP 合酶的亚基 B 的同源物，因此还显示了 NBD-Cl 与 Methanosarcina mazei Gö1 的 A-ATP 合酶的 B 之间的相互作用。数据显示，由于 NBD-Cl，B 的核苷酸结合减少，导致 MgATP-ATTO-647N 和 MgADP-ATTO-647N 的 IC（50）值分别为 41 μM 和 42 μM。

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The effect of NBD-Cl in nucleotide-binding of the major subunit alpha and B of the motor proteins F1FO ATP synthase and A1AO ATP synthase.

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本文引用的文献

[1]
Automated sample-changing robot for solution scattering experiments at the EMBL Hamburg SAXS station X33.

J Appl Crystallogr. 2008-8-16

[2]
Role of {alpha}-subunit VISIT-DG sequence residues Ser-347 and Gly-351 in the catalytic sites of Escherichia coli ATP synthase.

J Biol Chem. 2009-4-17

[3]
Three-dimensional structure of A1A0 ATP synthase from the hyperthermophilic archaeon Pyrococcus furiosus by electron microscopy.

J Biol Chem. 2009-4-10

[4]
A second transient position of ATP on its trail to the nucleotide-binding site of subunit B of the motor protein A(1)A(0) ATP synthase.

J Struct Biol. 2009-4

[5]
ATP synthase and the actions of inhibitors utilized to study its roles in human health, disease, and other scientific areas.

Microbiol Mol Biol Rev. 2008-12

[6]
Spectroscopic and crystallographic studies of the mutant R416W give insight into the nucleotide binding traits of subunit B of the A1Ao ATP synthase.

Proteins. 2009-6

[7]
New insights into structure-function relationships between archeal ATP synthase (A1A0) and vacuolar type ATPase (V1V0).

Bioessays. 2008-11

[8]
Life close to the thermodynamic limit: how methanogenic archaea conserve energy.

Results Probl Cell Differ. 2008

[9]
Synthesis, and structural and biological studies of efrapeptin C analogues.

Chem Biodivers. 2007-6

[10]
Cloning, purification, and nucleotide-binding traits of the catalytic subunit A of the V1VO ATPase from Aedes albopictus.

Protein Expr Purif. 2007-6

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