Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province - People's Republic of China.
Int J Biol Markers. 2009 Oct-Dec;24(4):271-6. doi: 10.1177/172460080902400409.
Quantum dots (QDs) as a biological labeling material for medical applications need to be evaluated for the sensitivity and stability of their fluorescence. Comparison of QD-based immunolabeling and commonly used immunohistochemical staining in detecting the expression of prostate stem cell antigen (PSCA) in bladder tumor tissues revealed that the two methods had similar sensitivity in the differential display of PSCA expression correlated with tumor stage and grade (kappa=0.92, p<0.001). In addition, the intensity of QD fluorescence remained stable for at least 10 days after conjugation to the PSCA protein and nearly 96% of the positive expression in samples lasted for one month.
量子点 (QD) 作为一种生物标记材料，在医学应用中需要评估其荧光的灵敏度和稳定性。比较基于 QD 的免疫标记和常用的免疫组织化学染色在检测膀胱肿瘤组织中前列腺干细胞抗原 (PSCA) 的表达，发现两种方法在显示与肿瘤分期和分级相关的 PSCA 表达方面具有相似的敏感性（kappa=0.92，p<0.001）。此外，QD 荧光的强度在与 PSCA 蛋白缀合后至少稳定 10 天，并且在一个月内，样本中近 96%的阳性表达持续存在。
