Suehiro Atsushi, Hirano Shigeru, Kishimoto Yo, Rousseau Bernard, Nakamura Tatsuo, Ito Juichi
Department of Otolaryngology-Head & Neck Surgery, Kyoto University, Japan.
Acta Otolaryngol. 2010 Jul;130(7):844-50. doi: 10.3109/00016480903426618.
Results of the current study revealed improved phonation threshold pressure (PTP), normalized mucosal wave amplitude (NMWA), and less contraction of the lamina propria in injured larynges treated with basic fibroblast growth factor (bFGF).
We investigated the effects of local injection of bFGF for treatment of acute vocal fold injury in a canine model.
Vocal folds of eight beagles were unilaterally injured by removal of the mucosa under direct laryngoscopy. Four beagles received local injections of bFGF delivered to the scarred vocal fold at 1 month after injury. The remaining four beagles received local injections of saline and served as a sham-treatment group. Larynges were harvested 5 months after treatment and excised larynx experiments were performed to measure PTP, NMWA, and normalized glottal gap (NGG). Histologic staining was performed to evaluate structural changes of the extracellular matrix.
Excised larynx measurements revealed significantly lower PTP and increased NMWA in bFGF-treated vocal fold. Elastica Van Gieson staining revealed less contraction of the bFGF-treated vocal fold. Histologic measurements revealed that the thickness of the lamina propria was significantly greater in the bFGF-treated vocal fold. Alcian blue staining revealed improved restoration of hyaluronic acid in the bFGF-treated vocal fold.
本研究结果显示，在接受碱性成纤维细胞生长因子（bFGF）治疗的损伤喉部中，发声阈压（PTP）改善、黏膜波振幅标准化值（NMWA）提高，固有层收缩减少。
我们在犬模型中研究了局部注射bFGF治疗急性声带损伤的效果。
在直接喉镜检查下通过去除黏膜对8只比格犬的声带进行单侧损伤。4只比格犬在损伤后1个月接受向瘢痕化声带局部注射bFGF。其余4只比格犬接受局部注射生理盐水并作为假治疗组。治疗5个月后取出喉部，进行切除喉部实验以测量PTP、NMWA和声门间隙标准化值（NGG）。进行组织学染色以评估细胞外基质的结构变化。
切除喉部测量显示，bFGF治疗的声带PTP显著降低，NMWA增加。弹性纤维范吉森染色显示bFGF治疗的声带收缩较少。组织学测量显示，bFGF治疗的声带固有层厚度显著更大。阿尔辛蓝染色显示bFGF治疗的声带透明质酸恢复改善。
