大鼠声带瘢痕切除喉模型
A rat excised larynx model of vocal fold scar.
作者信息
Welham Nathan V, Montequin Douglas W, Tateya Ichiro, Tateya Tomoko, Choi Seong Hee, Bless Diane M
机构信息
University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
出版信息
J Speech Lang Hear Res. 2009 Aug;52(4):1008-20. doi: 10.1044/1092-4388(2009/08-0049).
PURPOSE
To develop and evaluate a rat excised larynx model for the measurement of acoustic, aerodynamic, and vocal fold vibratory changes resulting from vocal fold scar.
METHOD
Twenty-four 4-month-old male Sprague-Dawley rats were assigned to 1 of 4 experimental groups: chronic vocal fold scar, chronic vocal fold scar treated with 100-ng basic fibroblast growth factor (bFGF), chronic vocal fold scar treated with saline (sham treatment), and unscarred untreated control. Following tissue harvest, histological and immunohistochemical data were collected to confirm extracellular matrix alteration in the chronic scar group; acoustic, aerodynamic, and high-speed digital imaging data were collected using an excised larynx setup in all groups. Phonation threshold pressure (P(th)), glottal resistance (R(g)), glottal efficiency (E(g)), vibratory amplitude, and vibratory area were used as dependent variables.
RESULTS
Chronically scarred vocal folds were characterized by elevated collagen Types I and III and reduced hyaluronic acid abundance. Phonation was achieved, and data were collected from all control and bFGF-treated larynges; however, phonation was not achieved with 3 of 6 chronically scarred and 1 of 6 saline-treated larynges. Compared with control, the chronic scar group was characterized by elevated P(th), reduced E(g), and intralarynx vibratory amplitude and area asymmetry. The bFGF group was characterized by P(th) below control-group levels, E(g) comparable with control, and vocal fold vibratory amplitude and area symmetry comparable with control. The sham group was characterized by P(th) comparable with control, E(g) superior to control, and vocal fold vibratory amplitude and area symmetry comparable with control.
CONCLUSIONS
The excised larynx model reported here demonstrated robust deterioration across phonatory indices under the scar condition and sensitivity to treatment-induced change under the bFGF condition. The improvement observed under the sham condition may reflect unanticipated therapeutic benefit or artifact. This model holds promise as a tool for the functional characterization of biomechanical tissue changes resulting from vocal fold scar and the evaluation of experimental therapies.
目的
建立并评估一种大鼠离体喉模型，用于测量声带瘢痕引起的声学、空气动力学及声带振动变化。
方法
将24只4月龄雄性Sprague-Dawley大鼠分为4个实验组之一：慢性声带瘢痕组、用100 ng碱性成纤维细胞生长因子（bFGF）治疗的慢性声带瘢痕组、用生理盐水治疗的慢性声带瘢痕组（假治疗组）和未瘢痕化未治疗的对照组。组织取材后，收集组织学和免疫组化数据以确认慢性瘢痕组细胞外基质的改变；所有组均使用离体喉装置收集声学、空气动力学和高速数字成像数据。将发声阈压力（P(th)）、声门阻力（R(g)）、声门效率（E(g)）、振动幅度和振动面积作为因变量。
结果
慢性瘢痕化声带的特征是I型和III型胶原蛋白升高，透明质酸丰度降低。所有对照组和bFGF治疗组的喉均实现发声并收集到数据；然而，6只慢性瘢痕化喉中有3只以及6只生理盐水治疗喉中有1只未实现发声。与对照组相比，慢性瘢痕组的特征是P(th)升高、E(g)降低以及喉内振动幅度和面积不对称。bFGF组的特征是P(th)低于对照组水平、E(g)与对照组相当，且声带振动幅度和面积对称性与对照组相当。假治疗组的特征是P(th)与对照组相当、E(g)优于对照组，且声带振动幅度和面积对称性与对照组相当。
结论
本文报道的离体喉模型显示，在瘢痕条件下，发声指标出现明显恶化，在bFGF条件下对治疗引起的变化敏感。在假治疗条件下观察到的改善可能反映了意外的治疗益处或假象。该模型有望成为一种工具，用于对声带瘢痕导致的生物力学组织变化进行功能表征以及评估实验性治疗方法。
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