Department of Otorhinolaryngology - Head and Neck Surgery, Inha University School of Medicine, 100 Inha-ro, Michuhol-gu, Incheon, 22212, Republic of Korea.
Department of Nanobiomedical Science, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam, 31116, Republic of Korea.
Tissue Eng Regen Med. 2020 Oct;17(5):651-658. doi: 10.1007/s13770-020-00280-6. Epub 2020 Jul 16.
Hepatocyte growth factor (HGF) has been shown to facilitate vocal fold (VF) wound healing. This study was undertaken to determine whether the therapeutic efficacy of HGF could be enhanced by applying it in hyaluronic acid and alginate (HA/ALG) composite hydrogels into VFs after injury in a rabbit model.
HGF was loaded into HA/ALG composite hydrogel (HGF-HA/ALG) and its in vitro release profile was evaluated. In addition, HGF-HA/ALG was injected into the VFs of rabbits immediately after direct injury and HGF or PBS was injected in the same manner into control groups. Macroscopic features were observed by endoscopy at 3 months post-injury. Functional analyses including mucosal waves of VFs and viscoelastic properties were performed by kymography following high-speed digital imaging and rheometer. Histopathological and immunohistochemical evaluations were also conducted on VFs.
HGF release from HGF-HA/ALG was sustained for up to 3 weeks. Rabbits treated with HGF-HA/ALG showed improved mucosal vibrations and VF viscoelastic properties as compared with the PBS and HGF controls. Histopathological staining revealed HGF-HA/ALG treated VFs showed less fibrosis than PBS and HGF controls, and immunohistochemical analysis demonstrated amounts of type I collagen and fibronectin were lower in HGF-HA/ALG treated animals than in PBS and HGF controls at 3 months post-injury.
HGF containing HA/ALG hydrogel enhanced healing in our rabbit model of VF injury.
肝细胞生长因子（HGF）已被证明可促进声带（VF）伤口愈合。本研究旨在确定在兔模型中，将 HGF 应用于受伤后的透明质酸和藻酸盐（HA/ALG）复合水凝胶中是否可以增强其治疗效果。
将 HGF 载入 HA/ALG 复合水凝胶（HGF-HA/ALG）中，并评估其体外释放情况。此外，在直接损伤后立即将 HGF-HA/ALG 注入兔 VF 中，并以相同方式将 HGF 或 PBS 注入对照组。在受伤后 3 个月，通过内窥镜观察宏观特征。通过高速数字成像和流变仪进行声门图分析，进行 VF 黏膜波和粘弹性特性的功能分析。还对 VF 进行了组织病理学和免疫组织化学评估。
HGF-HA/ALG 中的 HGF 释放可持续长达 3 周。与 PBS 和 HGF 对照组相比，接受 HGF-HA/ALG 治疗的兔子表现出改善的黏膜振动和 VF 粘弹性特性。组织病理学染色显示，与 PBS 和 HGF 对照组相比，HGF-HA/ALG 处理的 VF 纤维化程度较低，免疫组织化学分析显示，在受伤后 3 个月，HGF-HA/ALG 处理的动物中 I 型胶原蛋白和纤维连接蛋白的含量低于 PBS 和 HGF 对照组。
在我们的兔 VF 损伤模型中，含 HGF 的 HA/ALG 水凝胶增强了愈合。
