Dong Li, Tan Yuan, Ping Yu-jia, Zhu Nan-sun
Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Zhong Xi Yi Jie He Xue Bao. 2010 Jan;8(1):86-9. doi: 10.3736/jcim20100116.
To investigate the effects of different methods of treatment, including tonifying the kidney, activating blood and soothing the liver, on mice with autoimmune premature ovarian failure (POF).
After preparing ovarian antigen with the ovarian tissue of female BALB/C mice, POF in female BALB/C mice was induced by immunization injection of the ovarian antigen on multiple subcutaneous sites and two posterior soles. The mice were treated with Liuwei Dihuang Pill, Shaofu Zhuyu Granule, Chaihu Shugan Pill respectively after the third immunization. Levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E(2)), prolactin (PRL), testosterone (T), anti-ovarian antibodies (AOAb) and anti-zona pellucida antibody (aZP) in sera were analyzed by enzyme-linked immunosorbent assay.
Compared with the normal group, the levels of serum FSH and LH were increased and the level of E(2) was decreased in the untreated group. AOAb and aZP positive rates in the untreated group were obviously higher than those in the normal group. Liuwei Dihuang Pill could significantly reduce the levels of FSH and LH and increase the level of E(2) as compared with the untreated group, but there were no significant differences in the AOAb and aZP positive rates. Shaofu Zhuyu Granule and Chaihu Shugan Pill could significantly reduce the positive rates of AOAb and aZP as compared with the untreated group, but no significant effect on the levels of FSH, LH and E(2) were observed. The drugs had no effects on serum contents of PRL and T.
Tonifying the kidney method can recover the ovarian function in POF mice mainly by regulating the hormone levels. Activating blood and soothing the liver methods can improve the humoral immune function in POF mice.
探讨补肾、活血、疏肝等不同治疗方法对自身免疫性卵巢早衰（POF）小鼠的影响。
用雌性BALB/C小鼠卵巢组织制备卵巢抗原，通过在多个皮下部位及双侧后脚掌免疫注射卵巢抗原诱导雌性BALB/C小鼠发生POF。第三次免疫后分别给予六味地黄丸、少腹逐瘀颗粒、柴胡疏肝丸治疗。采用酶联免疫吸附测定法分析血清中卵泡刺激素（FSH）、黄体生成素（LH）、雌二醇（E₂）、催乳素（PRL）、睾酮（T）、抗卵巢抗体（AOAb）和抗透明带抗体（aZP）水平。
与正常组相比，未治疗组血清FSH和LH水平升高，E₂水平降低。未治疗组AOAb和aZP阳性率明显高于正常组。与未治疗组相比，六味地黄丸可显著降低FSH和LH水平，升高E₂水平，但AOAb和aZP阳性率差异无统计学意义。与未治疗组相比，少腹逐瘀颗粒和柴胡疏肝丸可显著降低AOAb和aZP阳性率，但对FSH、LH和E₂水平无明显影响。药物对血清PRL和T含量无影响。
补肾法主要通过调节激素水平恢复POF小鼠的卵巢功能。活血疏肝法可改善POF小鼠的体液免疫功能。
