Dong Li, Jiang Lin, Men Wei, Zhu Nan-sun
Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Zhong Xi Yi Jie He Xue Bao. 2008 Mar;6(3):294-7. doi: 10.3736/jcim20080315.
To investigate the preventive effect of Bushen Huoxue Recipe (BSHXR), a compound traditional Chinese herbal medicine, on autoimmune premature ovarian failure (POF) in mice.
Ovarian antigen was prepared with the ovarian tissue of female BALB/c mice. A mouse model of POF was established by immunization injection of the ovarian antigen of isotype female mice on multiple subcutaneous sites and two posterior soles. The POF mice were treated with BSHXR after the first and third immunization. The levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) in peripheral blood were detected by enzyme linked immunosorbent assay.
Lymphocyte infiltration was appeared in ovarian stroma of POF mice. The levels of FSH and LH were evaluated and the E(2) level was decreased significantly (P<0.05). BSHXR could reduce the increased levels of FSH and LH, increase the level of E(2) and the number of growing and mature follicles. The efficacy of early treatment was better than that of late treatment.
BSHXR can recover ovarian function in POF mice mainly by regulating the indiscriminate hormone level, and BSHXR has preventive effect on autoimmune POF in mice.
探讨复方中药补肾活血方（BSHXR）对小鼠自身免疫性卵巢早衰（POF）的预防作用。
取雌性BALB/c小鼠卵巢组织制备卵巢抗原。通过在多个皮下部位及双侧后足底注射同型雌性小鼠卵巢抗原进行免疫，建立POF小鼠模型。在首次和第三次免疫后用BSHXR对POF小鼠进行治疗。采用酶联免疫吸附测定法检测外周血中卵泡刺激素（FSH）、黄体生成素（LH）和雌二醇（E2）水平。
POF小鼠卵巢基质出现淋巴细胞浸润。FSH和LH水平升高，E2水平显著降低（P<0.05）。BSHXR可降低升高的FSH和LH水平，提高E2水平及生长和成熟卵泡数量。早期治疗效果优于晚期治疗。
BSHXR主要通过调节激素水平恢复POF小鼠卵巢功能，对小鼠自身免疫性POF具有预防作用。
