Cong Hailin, Xu Xiaodan, Yu Bing, Liu Huwei, Yuan Hua
College of Materials Science and Engineering, Qingdao University , Qingdao 266071, China.
Beijing National Laboratory for Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Biomicrofluidics. 2016 Mar 15;10(2):024107. doi: 10.1063/1.4943915. eCollection 2016 Mar.
A simple and effective universal serial bus (USB) flash disk type microfluidic chip electrophoresis (MCE) was developed by using poly(dimethylsiloxane) based soft lithography and dry film based printed circuit board etching techniques in this paper. The MCE had a microchannel diameter of 375 μm and an effective length of 25 mm. Equipped with a conventional online electrochemical detector, the device enabled effectively separation of bovine serum albumin, lysozyme, and cytochrome c in 80 s under the ultra low voltage from a computer USB interface. Compared with traditional capillary electrophoresis, the USB flash disk type MCE is not only portable and inexpensive but also fast with high separation efficiency.
本文利用基于聚二甲基硅氧烷的软光刻技术和基于干膜的印刷电路板蚀刻技术，开发了一种简单有效的通用串行总线（USB）闪存盘型微流控芯片电泳（MCE）。该MCE的微通道直径为375μm，有效长度为25mm。该装置配备了传统的在线电化学检测器，能够在超低电压下从计算机USB接口在80秒内有效分离牛血清白蛋白、溶菌酶和细胞色素c。与传统毛细管电泳相比，USB闪存盘型MCE不仅便携、廉价，而且速度快、分离效率高。
