Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA.
Anal Chim Acta. 2010 Jan 18;658(1):91-7. doi: 10.1016/j.aca.2009.10.058. Epub 2009 Oct 31.
Detection limits and reduced mobilities for 12 ribonucleotides and 4 ribonucleosides were measured by ambient pressure electrospray ionization-ion mobility spectrometry (ESI-IMS). With the instrument used in this study it was possible to separate some of these compounds within mixtures. Detection limits reported for ribonucleotides and ribonucleosides ranged from 15 to 300 pmol and the reduced mobilities ranged from 41 to 56 suggesting that ambient pressure ESI-IMS may be used for their rapid and sensitive separation and detection. This report demonstrates that it was possible to use ion mobility spectrometry (IMS) to obtain a spectrum for the separation of nucleotides and nucleosides in less than 1 min. The application holds great promise for nucleotide analysis in the area of separating DNA fragments in genome sequencing and also for forensics DNA typing examinations used for the identification of blood stains in crime scenes and paternity testing.
采用常压电喷雾离子化-离子淌度谱法（ESI-IMS）测定了 12 种核糖核苷酸和 4 种核糖核苷的检测限和迁移率降低值。在这项研究中使用的仪器能够在混合物中分离出其中的一些化合物。报告的核糖核苷酸和核糖核苷的检测限范围为 15 至 300 pmol，迁移率降低值范围为 41 至 56，表明常压 ESI-IMS 可用于它们的快速和灵敏分离和检测。本报告表明，有可能使用离子淌度谱法（IMS）在不到 1 分钟的时间内获得核苷酸和核苷分离的谱图。该应用在基因组测序中分离 DNA 片段、法医 DNA 分型检测用于识别犯罪现场血迹和亲子鉴定方面的核苷酸分析领域具有广阔的应用前景。
