Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
J Am Soc Mass Spectrom. 2011 Sep;22(9):1477-85. doi: 10.1007/s13361-011-0179-8. Epub 2011 Jul 9.
A new instrument that combines ion mobility spectrometry (IMS) separations with tandem mass spectrometry (MS(n)) is described. Ion fragmentation is achieved with vacuum ultraviolet photodissociation (VUV PD) and/or collision-induced dissociation (CID). The instrument is comprised of an approximately 1 m long drift tube connected to a linear trap that has been interfaced to a pulsed F(2) laser (157 nm). Ion gates positioned in the front and the back of the primary drift region allow for mobility selection of specific ions prior to their storage in the ion trap, mass analysis, and fragmentation. The ion characterization advantages of the new instrument are demonstrated with the analysis of the isomeric trisaccharides, melezitose and raffinose. Mobility separation of precursor ions provides a means of separating the isomers and subsequent VUV PD generates unique fragments allowing them to be distinguished.
一种新型仪器，将离子淌度谱（IMS）分离与串联质谱（MS(n)）相结合，本文对此加以描述。离子碎片化是通过真空紫外光解（VUV PD）和/或碰撞诱导解离（CID）来实现的。该仪器由一个大约 1 米长的漂移管连接到一个线性阱，该线性阱已经与脉冲 F2 激光（157nm）接口。位于主漂移区前后的离子门允许在将特定离子存储在离子阱中、进行质量分析和碎片化之前，对其进行迁移率选择。新型仪器的离子特性优势通过对异构三糖，蜜二糖和棉子糖的分析得到了证明。前体离子的迁移率分离提供了一种分离异构体的方法，随后的 VUV PD 生成独特的片段，使它们能够被区分开来。
