Zhang Zhu, Zhou Bin, Zhang Jian, Chen Yue, Lai Ting, Yan Li, Liang Ailing, Li Yi, Wang Yanyun, Chen Yu, Zhang Lin, Xi Ming-Rong
Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Cancer Genet Cytogenet. 2010 Jan 15;196(2):146-52. doi: 10.1016/j.cancergencyto.2009.09.006.
Among gynecological malignancies, ovarian cancer is the leading cause of death. The overall 5-year survival rate remains poor, and the pathogenesis is unknown. The interleukin-23 receptor (IL23R) is known to be critically involved in the carcinogenesis of different malignant tumors. To assess the role of IL23R in ovarian cancer, we conducted a study to investigate the polymorphisms of the IL23R gene in 96 Han Chinese women with histologically proven ovarian cancer. Polymerase chain reaction-restriction fragment length polymorphism was used for genotyping. In all three single nucleotide polymorphisms of IL23R studied, the distribution of genotype and allele frequencies of rs10889677 differed significantly between patients and controls. The frequency of allele C of rs10889677 was significantly increased in cases compared with controls (0.281 vs. 0.183, odds ratio OR=1.752, 95% confidence interval CI=1.107-2.772). Furthermore, when stratified by tumor stage, we found that the allele frequencies of rs11465817 differed significantly between FIGO stage I+II and III+IV. The higher frequency of allele A was significantly associated with advanced ovarian cancer (P=0.027, OR=2.087, 95% CI=1.083-4.023). These findings indicate that IL23R polymorphisms may play an important role in the susceptibility and prognosis of ovarian cancer in the Chinese population.
在妇科恶性肿瘤中，卵巢癌是主要的死亡原因。总体5年生存率仍然很低，其发病机制尚不清楚。已知白细胞介素-23受体（IL23R）在不同恶性肿瘤的致癌过程中起关键作用。为了评估IL23R在卵巢癌中的作用，我们进行了一项研究，调查96例经组织学证实为卵巢癌的汉族女性中IL23R基因的多态性。采用聚合酶链反应-限制性片段长度多态性方法进行基因分型。在所研究的IL23R的所有三个单核苷酸多态性中，rs10889677的基因型和等位基因频率分布在患者和对照组之间存在显著差异。与对照组相比，rs10889677的等位基因C频率在病例组中显著增加（0.281对0.183，优势比OR=1.752，95%置信区间CI=1.107-2.772）。此外，按肿瘤分期分层时，我们发现rs11465817的等位基因频率在国际妇产科联盟（FIGO）I+II期和III+IV期之间存在显著差异。等位基因A的较高频率与晚期卵巢癌显著相关（P=0.027，OR=2.087，95%CI=1.083-4.023）。这些发现表明，IL23R基因多态性可能在中国人群卵巢癌的易感性和预后中起重要作用。
