Department of Chemistry, University of Warsaw, 02-093 Warsaw, Poland.
Anal Biochem. 2010 May 1;400(1):151-3. doi: 10.1016/j.ab.2010.01.016. Epub 2010 Jan 18.
A one-step carbodiimide method was found to allow covalent binding of enzymes to the inner wall of poly(vinyl chloride) (PVC) tubing. The immobilization is performed under mild conditions without laborious pretreatment or activation of reactor surface. In these preliminary studies, alkaline phosphatase (ALP, EC 3.1.3.1) and p-nitrophenyl phosphate (NPP) were applied as a model enzyme and substrate, respectively. The resulting open-tubular bioreactor exhibits satisfactory operational and storage stability. In addition, a novel and very simple instrumental concept for optical monitoring of the biocatalytic process directly inside the microbioreactor using a system of paired emitter-detector diodes is presented.
一步法碳二亚胺法被发现可以使酶共价结合到聚氯乙烯（PVC）管的内壁上。该固定化在温和的条件下进行，不需要繁琐的预处理或反应器表面的活化。在这些初步研究中，碱性磷酸酶（ALP，EC 3.1.3.1）和对硝基苯磷酸酯（NPP）分别用作模型酶和底物。所得的开管生物反应器表现出令人满意的操作和储存稳定性。此外，还提出了一种新颖而非常简单的仪器概念，用于使用一对发射器-检测器二极管系统直接在微生物反应器内对生物催化过程进行光学监测。
