Pokrzywnicka Marta, Koncki Robert, Tymecki Łukasz
Department of Chemistry, University of Warsaw, Pasteura 1, 02-093, Warsaw, Poland.
Anal Bioanal Chem. 2015 Mar;407(7):1807-12. doi: 10.1007/s00216-014-8434-z. Epub 2015 Jan 27.
Integration of immobilized enzymes with light-emitting diodes (LEDs) leads to the development of optoelectronic enzyme-based biosensors. In this work, urease, used as a model enzyme, immobilized in the form of an open-tubular microbioreactor or biosensing membrane that has been integrated with two red LEDs. It forms complete, fiberless, miniaturized, and extremely economic biooptoelectronic devices useful for nonstationary measurements under flow analysis conditions. Both enzyme-based biodevices, operating according to the paired emitter detector diode (PEDD) principle, allow relatively fast, highly sensitive, and well-reproducible urea detection in the millimolar range of concentrations. Potential analytical applications of the developed urea bioPEDDs have been announced. Both presented constructions will be easily adapted for the development of other optoelectronic biosensors exploring various enzyme-based schemes of biodetection.
固定化酶与发光二极管（LED）的整合导致了基于光电子酶的生物传感器的发展。在这项工作中，脲酶作为一种模型酶，以开放管式微生物反应器或生物传感膜的形式固定化，并与两个红色LED整合。它形成了完整、无纤维、小型化且极其经济的生物光电子器件，可用于流动分析条件下的非稳态测量。这两种基于酶的生物器件均根据配对发射极探测器二极管（PEDD）原理运行，能够在毫摩尔浓度范围内相对快速、高度灵敏且可重复地检测尿素。已公布了所开发的尿素生物PEDD的潜在分析应用。所展示的两种结构都将很容易适用于开发其他基于光电子的生物传感器，这些传感器探索各种基于酶的生物检测方案。
