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从燕麦中提取和表征β-葡聚糖用于工业利用。

Extraction and characterization of beta-D-glucan from oat for industrial utilization.

机构信息

Department of Food Technology, PMAS Arid Agriculture University Rawalpindi, Pakistan.

出版信息

Int J Biol Macromol. 2010 Apr 1;46(3):304-9. doi: 10.1016/j.ijbiomac.2010.01.002. Epub 2010 Jan 18.

DOI:10.1016/j.ijbiomac.2010.01.002
PMID:20083136
Abstract

Oat beta-D-glucan is a valuable functional ingredient having numerous industrial, nutritional and health benefits. Its extraction needs careful attention as extraction process may affect the physiochemical and functional properties of extracted beta-D-glucan. The present study aimed at analyzing the effect of extraction of beta-D-glucan gum pellets from oat cultivar followed by detailed chemical and functional analysis. Enzymatic extraction process resulted in highest yield and recovery. Chemical analysis revealed protein as a dominating impurity. The water binding capacity of the beta-D-glucan ranged between 3.14 and 4.52 g g(-1) of sample. beta-D-Glucan exhibited ideal foaming stability when appropriate extraction technique was used. The viscosity of beta-D-glucan gum ranged between 35.6 and 56.16 cp. The color analysis showed L* value of beta-D-glucan gum pellet ranged between 72.18 and 83.54. Phosphorus, potassium and calcium appeared as major minerals in beta-D-glucan gum whereas iron, manganese and copper appeared as minor minerals. FTIR spectroscopy also confirms the presence of beta-D-glucan, protein and other components in extracted beta-D-glucan gum pellets. Overall, extracted beta-D-glucan showed a good potential for industrial usage.

摘要

燕麦β-葡聚糖是一种具有多种工业、营养和健康益处的有价值的功能性成分。由于提取过程可能会影响提取的β-葡聚糖的物理化学和功能特性,因此需要仔细注意其提取。本研究旨在分析从燕麦品种中提取β-葡聚糖胶粒的效果,然后进行详细的化学和功能分析。酶提取工艺的得率和回收率最高。化学分析表明蛋白质是主要的杂质。β-葡聚糖的持水能力在 3.14 到 4.52 g g(-1)之间。当使用适当的提取技术时,β-葡聚糖表现出理想的泡沫稳定性。β-葡聚糖胶的粘度在 35.6 到 56.16 cp 之间。颜色分析表明β-葡聚糖胶粒的 L*值在 72.18 到 83.54 之间。β-葡聚糖胶中磷、钾和钙是主要的矿物质,而铁、锰和铜是次要的矿物质。傅里叶变换红外光谱(FTIR)也证实了提取的β-葡聚糖胶粒中存在β-葡聚糖、蛋白质和其他成分。总的来说,提取的β-葡聚糖具有很好的工业应用潜力。

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