Unidad de Sistemas Acuáticos, Centro EULA-Chile, Universidad de Concepción, Barrio Universitario S/N, Concepción, Chile.
Ecotoxicol Environ Saf. 2010 May;73(4):515-23. doi: 10.1016/j.ecoenv.2009.12.004. Epub 2010 Jan 18.
This study aimed to analyze oxidative stress parameters, including levels of the antioxidant glutathione (GSH), activity of glutamate-cysteine ligase (GCL) and glutathione-S-transferase (GST), total antioxidant capacity and protein oxidation, in the polychaete Perinereis gualpensis (Nereididae) collected from the Biobío, Itata, Valdivia and Lingue estuaries in Chile, which present different degrees of anthropogenic pressure. Sampling sites were characterized considering a geographic information system and the physicochemical characteristics of water and sediment. Significant differences (p<0.05) were observed between the sampling sites for most of the responses (GSH, GCL, GST and antioxidant capacity), mainly related to human activities such as agriculture, industry, among others. Multivariate correlation analysis indicates a certain relationship of antioxidant responses with human activities, salinity, and worm weight, this last employed to standardize GST and antioxidant capacity. These results clearly indicate biomarker responses in P. gualpensis in Biobío and Valdivia estuaries, the more affected by human activities.
本研究旨在分析多毛类环节动物海蚯蚓（Nereididae 科）体内的氧化应激参数，包括抗氧化剂谷胱甘肽（GSH）水平、谷胱甘肽合成酶（GCL）和谷胱甘肽-S-转移酶（GST）活性、总抗氧化能力和蛋白质氧化程度。这些海蚯蚓样本分别取自智利比奥比奥、伊塔塔、瓦尔迪维亚和灵圭这四个河口，这些河口受到不同程度的人为压力。通过地理信息系统对采样点进行了特征描述，并对水和沉积物的理化特性进行了分析。结果表明，大多数响应（GSH、GCL、GST 和抗氧化能力）在采样点之间存在显著差异（p<0.05），这主要与农业、工业等人类活动有关。多变量相关分析表明，抗氧化反应与人类活动、盐度和虫体重量之间存在一定的关系，后两者用于 GST 和抗氧化能力的标准化。这些结果清楚地表明，比奥比奥和瓦尔迪维亚河口的海蚯蚓存在生物标志物反应，这些河口受到人类活动的影响更大。
