Aquatic Systems Research Unit, EULA Chile Environmental Sciences Centre, Universidad de Concepción, Chile.
Ecotoxicol Environ Saf. 2013 Mar;89:182-8. doi: 10.1016/j.ecoenv.2012.11.026. Epub 2012 Dec 27.
In situ translocation experiments are advantageous relative to traditional laboratory experiments, particularly for understanding the bioavailability of trace metals like mercury (Hg). Individuals of the polychaete Perinereis gualpensis were translocated from a reference site (Raqui estuary, Chile) to an estuarine site with significant sediment Hg concentrations (Lenga estuary: 1.78-9.89 mg/kg). Individuals were exposed in polluted and non-polluted sediments for 21 days and sampled every 7 days with cages deployed at three different depths. Tissue Hg concentrations were measured in conjunction with oxidative stress responses. Translocated polychaetes rapidly accumulated Hg. Glutathione S-transferase (GST) activities measured from posterior body regions were 2-fold higher than control activities after 21 days of exposure. Other antioxidant measures were idiosyncratic. Distinct burrowing behavior differences were observed; control polychaetes exhibited more homogenous vertical distributions, whereas in Lenga, worms tended to remain in upper layers. These studies demonstrate that under natural conditions, Hg is highly bioavailable to polychaetes affecting both biochemical and behavioral responses after relatively short-term exposure.
在原地迁移实验相对于传统的实验室实验具有优势，特别是对于理解痕量金属（如汞）的生物可利用性。多毛类动物 Perinereis gualpensis 的个体从参考地点（智利 Raqui 河口）转移到具有显著沉积物汞浓度的河口地点（Lenga 河口：1.78-9.89 mg/kg）。个体在污染和未污染的沉积物中暴露 21 天，并每隔 7 天用笼子在三个不同深度进行采样。测量组织中的汞浓度与氧化应激反应。迁移的多毛类动物迅速积累汞。暴露 21 天后，后体区域测量的谷胱甘肽 S-转移酶（GST）活性比对照活性高 2 倍。其他抗氧化措施则各有特点。观察到明显的挖掘行为差异；对照多毛类动物表现出更均匀的垂直分布，而在 Lenga，蠕虫往往停留在上层。这些研究表明，在自然条件下，Hg 对多毛类动物具有高度的生物可利用性，在相对短期暴露后会影响生物化学和行为反应。
