Moradzadeh Arash, Brenner Michael J, Whitlock Elizabeth L, Tong Alice Y, Luciano Janina P, Hunter Daniel A, Myckatyn Terence M, Mackinnon Susan E
Department of Otolaryngology-Head & Neck Surgery, Washington University School of Medicine, St Louis, Missouri, USA.
Arch Facial Plast Surg. 2010 Jan-Feb;12(1):40-7. doi: 10.1001/archfacial.2009.104.
To determine the Sunderland classification of a bipolar electrocautery injury.
Twenty-two rats received crush (a reproducible Sunderland second-degree injury) or bipolar electrocautery injury and were evaluated for functional, histomorphometric, and immunohistochemical recovery at 21 or 42 days. Animal experiments were performed between July 3 and December 12, 2007. Axonal regeneration and end plate reinnervation were evaluated in double transgenic cyan fluorescent protein-conjugated Thy1 and green fluorescent protein-conjugated S100 mice.
Compared with crush injury, bipolar electrocautery injury caused greater disruption of myelin and neurofilament architecture at the injury site and decreased nerve fiber counts and percentage of neural tissue distal to the injury (P =.007). Complete functional recovery was seen after crush but not bipolar electrocautery injury. Serial live imaging demonstrated axonal regeneration at week 1 after crush and at week 3 after bipolar electrocautery injury. Qualitative assessment of motor end plate reinnervation at 42 days demonstrated complete neuromuscular end plate reinnervation in the crush group and only limited reinnervation in the bipolar electrocautery group.
Bipolar electrocautery injury in a rodent model resulted in a Sunderland third-degree injury, characterized by gradual, incomplete recovery without intervention.
确定双极电凝损伤的桑德兰分类。
22只大鼠接受挤压伤（一种可重复性的桑德兰二级损伤）或双极电凝损伤，并在21天或42天时进行功能、组织形态计量学和免疫组织化学恢复评估。动物实验于2007年7月3日至12月12日进行。在双转基因青色荧光蛋白偶联的Thy1和绿色荧光蛋白偶联的S100小鼠中评估轴突再生和终板再支配情况。
与挤压伤相比，双极电凝损伤导致损伤部位的髓鞘和神经丝结构破坏更严重，损伤远端的神经纤维数量和神经组织百分比降低（P = 0.007）。挤压伤后可见完全功能恢复，而双极电凝损伤后未见。连续活体成像显示挤压伤后第1周和双极电凝损伤后第3周有轴突再生。42天时对运动终板再支配的定性评估显示，挤压伤组神经肌肉终板完全再支配，而双极电凝组仅有有限的再支配。
啮齿动物模型中的双极电凝损伤导致桑德兰三级损伤，其特征为未经干预时恢复缓慢且不完全。
