Division of Plant Microbe Interactions, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India.
Arch Microbiol. 2010 Mar;192(3):185-93. doi: 10.1007/s00203-010-0544-1.
Currently, it is presumed that Escherichia coli is not a normal inhabitant of the soil. Soilborne E. coli strains were isolated from broad range of 7 geoclimatic zones of India, indicating that E. coli can survive and thrive under different extreme soil conditions. Diversity among E. coli strains from widely separated geographic regions using enterobacterial repetitive intergenic consensus (ERIC)-PCR did not reveal any relationships between the genotypes and the source of isolation. Inoculation of maize (Zea mays cv. Arkil) seeds with E. coli NBRIAR3 (NBRIAR3) significantly enhanced (P < 0.05) plant growth and nutrient uptake, when compared with uninoculated control. Presence or absence of NBRIAR3 did not affect significantly (P < 0.05) diversity indexes, using substrate utilization patterns on the Biolog Eco plates. Clone libraries based on 16S rRNA gene from rhizosphere of maize plants demonstrated rather similar phylotype diversity from the uninoculated control and NBRIAR3-treated rhizosphere soil, which further indicated that NBRIAR3 did not exert a major influence on the overall bacterial diversity. The methodological approach described in this study supports the idea that E. coli should be treated as native soil bacterium instead of as an "indicator" of the possible presence of other fecal coliform bacteria.
The online version of this article (doi:10.1007/s00203-010-0544-1) contains supplementary material, which is available to authorized users.
目前，人们认为大肠杆菌不是土壤的正常居民。从印度 7 个地理气候带的广泛范围内分离出了土壤大肠杆菌菌株，这表明大肠杆菌可以在不同的极端土壤条件下生存和繁殖。使用肠细菌重复基因间一致性（ERIC）-PCR 对来自广泛分离的地理区域的大肠杆菌菌株进行多样性分析，并未发现基因型与分离源之间存在任何关系。与未接种对照相比，用大肠杆菌 NBRIAR3（NBRIAR3）接种玉米（Zea mays cv. Arkil）种子可显著提高（P<0.05）植物生长和养分吸收。NBRIAR3 的存在与否对基于 Biolog Eco 板上底物利用模式的多样性指数没有显著影响（P<0.05）。基于玉米根际土壤 16S rRNA 基因的克隆文库显示，未接种对照和 NBRIAR3 处理的根际土壤的系统发育多样性相当相似，这进一步表明 NBRIAR3 对整体细菌多样性没有产生重大影响。本研究中描述的方法支持这样一种观点，即应该将大肠杆菌视为原生土壤细菌，而不是其他粪便大肠菌群可能存在的“指示物”。
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