Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, Prague 6, 16206 Czech Republic.
J Tissue Eng Regen Med. 2010 Aug;4(6):454-63. doi: 10.1002/term.256.
Preparation of soft poly(amino acid) hydrogels containing biomimetic cell-adhesive peptides was investigated. Covalently crosslinked gels were formed by radical co-polymerization of methacryloylated macromonomer poly[N(5)-(2-hydroxyethyl)-L-glutamine-stat-L-alanine-stat-methacryloyllysine] with 2-hydroxyethyl methacrylate (HEMA) as minor co-monomer. Hydrogels carrying biomimetic peptides were prepared by using methacryloylated peptides, such as methacryloyl-GGGRGDSG-OH and methacryloyl-GGGYIGSR-OH, as additional monomers in the polymerization mixture. Mechanical stability and swelling in water of the hydrogels obtained for different solid:water and polypeptide:HEMA ratios were evaluated. The microporosity of gels (5-20 microm), dependent on the polyHEMA phase separation in water, was followed by low-vacuum SEM. The effect of biomimetic modification of hydrogels with RGDS and YIGSR peptides on the seeding efficiency of porcine mesenchymal stem cells (MSCs) was studied in vitro. While unmodified hydrogels showed very low cell adhesion, due to their highly hydrophilic nature, the incorporation of adhesive peptides significantly improved the adhesion and viability of seeded cells.
研究了含有仿生细胞黏附肽的软聚（氨基酸）水凝胶的制备。通过自由基共聚，将甲基丙烯酰化大分子单体聚[N(5)-(2-羟乙基)-L-谷氨酸酯-Stat-L-丙氨酸酯-Stat-甲基丙烯酰基赖氨酸]与 2-羟乙基甲基丙烯酸酯（HEMA）作为少量共聚单体交联，形成共价交联凝胶。通过使用甲基丙烯酰化肽，如甲基丙烯酰基-GGGRGDSG-OH 和甲基丙烯酰基-GGGYIGSR-OH，作为聚合混合物中的附加单体，制备了携带仿生肽的水凝胶。评估了不同固含量：水含量和多肽：HEMA 比的水凝胶的机械稳定性和在水中的溶胀度。凝胶的微孔性（5-20μm），取决于在水中的聚 HEMA 相分离，通过低真空 SEM 进行了跟踪。研究了 RGDS 和 YIGSR 肽对仿生修饰水凝胶对猪间充质干细胞（MSCs）接种效率的影响。未修饰的水凝胶由于其高度亲水性，细胞黏附性非常低，而掺入黏附肽则显著提高了接种细胞的黏附和活力。
