Department of Chemical and Materials Engineering, University of Kentucky, Lexington, 40506, USA.
Acta Biomater. 2011 May;7(5):1956-64. doi: 10.1016/j.actbio.2011.01.024. Epub 2011 Jan 18.
Biodegradable hydrogels have been studied extensively in recent years for biomedical applications, including tissue engineering and drug delivery, due to their tunable properties and similarities to natural soft tissue. In this work, two poly(β-amino ester) biodegradable hydrogel systems were synthesised and studied in vitro. Different degradation profiles were observed, ranging from 7 h to 4 months, and the compressive modulus was shown to decrease as degradation proceeded. MTT cytotoxicity analysis was used to analyze the cellular response to the degradation products, and the hydrogel systems exhibited similar toxicity to poly(dl-lactide-co-glycolide) degradation products. Finally, cell attachment was studied by seeding pluripotent mesenchymal cells directly onto the hydrogel surfaces followed by a live/dead assay and fluorescent imaging. Cells showed significant viability at 24h (98%) and slightly lower, but still substantial viability, at 48 h (72%). These hydrogels exhibited a range of properties and favorable cellular responses, all which indicate that these materials could be viable materials for tissue regeneration and other biomedical applications.
近年来，可生物降解水凝胶因其可调的性质和与天然软组织的相似性，在生物医学应用中，包括组织工程和药物输送方面，得到了广泛的研究。在这项工作中，我们合成了两种聚（β-氨基酯）可生物降解水凝胶系统，并在体外进行了研究。观察到不同的降解曲线，降解时间从 7 小时到 4 个月不等，随着降解的进行，压缩模量逐渐降低。MTT 细胞毒性分析用于分析细胞对降解产物的反应，结果表明，水凝胶系统的毒性与聚（DL-丙交酯-共-乙交酯）降解产物相似。最后，通过直接将多能间充质细胞接种到水凝胶表面，然后进行死活染色和荧光成像，研究了细胞的附着情况。细胞在 24 小时时表现出显著的活力（98%），在 48 小时时略有降低，但仍具有很高的活力（72%）。这些水凝胶表现出一系列的性质和良好的细胞反应，所有这些都表明这些材料可能是组织再生和其他生物医学应用的可行材料。
