Andresiuk M V, Gordo F Ponce, Bandera C Cuesta, Elissondo M C, Dopchiz M, Denegri G
Laboratorio de Zoonosis Parasitarias, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes, Buenos Aires, Argentina.
Rev Argent Microbiol. 2009 Oct-Dec;41(4):218-25.
In the present study we have compared cattle isolates of Echinococcus granulosus from Argentina and Spain. The aim was to compare and determine if there exist phenotypic and genetic differences within E. granulosus cattle isolates between an endemic area of Spain (where the disease is mainly restricted to a sheep-dog cycle) and an endemic area of Argentina (where cattle are the most abundant intermediate hosts). The Spanish samples were previously identified as G1 genotype. The Argentinean samples were also identified as G1, but some variants were found for the cytochrome c oxidase-1 (CO1) and NADH dehydrogenase-1 (ND1) mitochondrial genes. When comparing the cyst features and the morphology of the larval rostellar hooks in both regions, some differences were found. The morphometric analyses of the larval rostellar hooks showed the existence of two distinct clearly separated groups (one corresponding to the Argentinean samples and the other to the Spanish ones). In conclusion, there are some genetic and phenotypic differences within E. granulosus cattle isolates from Argentina and Spain. Probably these differences, more important from an epidemiological point of view, are related to different steps in the disease control in both countries. Further studies involving other epidemiological, morphometric and molecular data, including other types of livestock, would contribute to clarify and expand the present work.
在本研究中，我们比较了来自阿根廷和西班牙的细粒棘球绦虫牛分离株。目的是比较并确定在西班牙的一个地方性流行区（该病主要局限于羊 - 犬循环）和阿根廷的一个地方性流行区（牛是最主要的中间宿主）的细粒棘球绦虫牛分离株之间是否存在表型和遗传差异。西班牙的样本先前被鉴定为G1基因型。阿根廷的样本也被鉴定为G1，但细胞色素c氧化酶 - 1（CO1）和烟酰胺腺嘌呤二核苷酸脱氢酶 - 1（ND1）线粒体基因发现了一些变异。在比较两个地区的囊肿特征和幼虫顶突小钩的形态时，发现了一些差异。幼虫顶突小钩的形态计量学分析显示存在两个明显分开的不同组（一组对应阿根廷的样本，另一组对应西班牙的样本）。总之，来自阿根廷和西班牙的细粒棘球绦虫牛分离株之间存在一些遗传和表型差异。从流行病学角度来看，这些差异可能更重要，与两国疾病控制的不同阶段有关。涉及其他流行病学、形态计量学和分子数据（包括其他类型家畜）的进一步研究将有助于阐明和扩展目前的工作。
