Bhattacharya D, Bera A K, Bera B C, Maity A, Das S K
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700037, India.
Vet Parasitol. 2007 Feb 28;143(3-4):371-4. doi: 10.1016/j.vetpar.2006.09.007. Epub 2006 Oct 9.
Twelve isolates of Echinococcus granulosus, collected from domestic animals, including cattle, buffalo and sheep were analysed for DNA nucleotide sequence variation within mitochondrial cytochrome c oxidase I (coxI), NADH dehydrogenase subunit I (nadI) and internal transcribed spacer gene I (ITS1). After analysis of sequence information this was found that the fragment size of ITS1 of buffalo isolate was more in comparison to cattle and sheep isolates. Based on the nadI genotype this was found that Indian cattle, buffalo and sheep isolates could be grouped into E. granulosus sensu stricto. Based on coxI genotype two sheep isolates and one buffalo isolate were homologous to G2 genotype. Rests of the isolates were microvariants of G2 genotype. Presence of G2 genotype in buffalo is the first report of this genotype from this host.
对从家畜（包括牛、水牛和绵羊）采集的12株细粒棘球绦虫分离株进行了线粒体细胞色素c氧化酶I（coxI）、NADH脱氢酶亚基I（nadI）和内部转录间隔区基因I（ITS1）内的DNA核苷酸序列变异分析。在分析序列信息后发现，水牛分离株的ITS1片段大小比牛和绵羊分离株的更大。基于nadI基因型，发现印度牛、水牛和绵羊分离株可归为狭义细粒棘球绦虫。基于coxI基因型，两株绵羊分离株和一株水牛分离株与G2基因型同源。其余分离株为G2基因型的微变体。水牛中存在G2基因型是该宿主中该基因型的首次报道。
