Voznesenskaia O S, Sharpatyĭ V A
Radiobiologiia. 1991 Jan-Feb;31(1):52-8.
The methods of gel electrophoresis and spectrophotometry were used to study DNA destruction in the chromatin (a nucleosome set of 1 to 8 units) irradiated with 60Co-gamma quanta in water solutions at doses ranging from 1 to 40 kGy, depending on the chromatin concentration, the nature of gas that saturated the solution (Ar, N2O), and the protein/DNA ratio in the chromatin samples. In conditions of a complete involvement of water radiolysis products, OH and eaq, in the reaction with the chromatin (the chromatin concentrations in the solution exceeding 0.04 weight % when calculated per DNA) determined were G ("DNA-protein") cross-links = 6.10(-5), the G (chromophoric group degradation) = 0.4, and G (double-strand breaks) less than 10(-5), as well as G of the process of free DNA release into the solution. The latter depend upon the protein/DNA ratio in the sample and increased with increasing protein content of the chromatin with the protein/DNA ratio being greater than 1.1/1.
采用凝胶电泳和分光光度法，研究了在水溶液中，当染色质（1至8个单位的核小体组）受到剂量范围为1至40千戈瑞的60Co-γ量子照射时的DNA破坏情况，该破坏情况取决于染色质浓度、溶液中饱和气体的性质（氩气、一氧化二氮）以及染色质样品中的蛋白质/DNA比率。在水辐射分解产物OH和水合电子完全参与与染色质反应的条件下（当按DNA计算时，溶液中染色质浓度超过0.04重量%），测定得到G（“DNA-蛋白质”交联）=6.10(-5)，G（发色团降解）=0.4，G（双链断裂）小于10(-5)，以及游离DNA释放到溶液中的过程的G值。后者取决于样品中的蛋白质/DNA比率，并随着染色质蛋白质含量的增加而增加，蛋白质/DNA比率大于1.1/1。
