Center for Resuscitation Science, Department of Emergency Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Langmuir. 2010 Feb 16;26(4):2452-9. doi: 10.1021/la903703u.
Important physiological, pathological, and technological processes occur at continuous and dispersed phase interfaces. Understanding these processes is limited by inability to quantitate molecular events occurring at the interface. To provide a model-independent measurement of protein concentration and mobility at the interface, we employed confocal laser scanning microscopy (CLSM). Fluorescently labeled albumin and fibrinogen were studied singly, pairwise, and with a surfactant, Pluronic F-127, in aqueous droplets. CLSM enables measurement of molecular behaviors manifest as surface inhomogeneity and of biophysical quantities including partitioning between the bulk and the gas-liquid (GL) interface. We conclude that albumin and fibrinogen behave substantially differently at the GL interface, adsorption from multispecies solutions is fundamentally different than adsorption from solutions of single species, and surfactants can inhibit proteins from occupying the interface.
重要的生理、病理和技术过程发生在连续相与分散相的界面处。由于无法定量分析界面处发生的分子事件，因此对这些过程的理解受到限制。为了在不依赖模型的情况下测量界面处的蛋白质浓度和迁移率，我们采用了共聚焦激光扫描显微镜（CLSM）。单独、成对以及与表面活性剂 Pluronic F-127 一起研究了荧光标记的白蛋白和纤维蛋白原在水相液滴中的情况。CLSM 可用于测量表现为表面不均匀性的分子行为，以及包括在主体和气液（GL）界面之间分配在内的生物物理量。我们得出结论，白蛋白和纤维蛋白原在 GL 界面处的行为有很大的不同，从多物种溶液中的吸附与从单物种溶液中的吸附根本不同，并且表面活性剂可以抑制蛋白质占据界面。
