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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Cain S T, Nemeroff C B

Department of Psychiatry, Duke University Medical Center, Durham, NC.

Prog Neuropsychopharmacol Biol Psychiatry. 1991;15(1):83-9. doi: 10.1016/0278-5846(91)90043-z.

Caudate nucleus slices from rat brain were prepared and incubated with 5 microM neurotensin for 30 seconds and 5 minutes. Following homogenization of the caudate nucleus slices, proteins were phosphorylated in vitro in the presence of CaCl2 or cyclic AMP. Phosphorylated proteins were separated by electrophoresis, and phosphate incorporation into individual proteins quantitated by microdensitometry of the resultant autoradiographs. 2. Incubation of caudate nucleus slices with neurotensin altered the subsequent in vitro calcium-dependent phosphorylation of several specific protein substrates, but in contrast, incubation with neurotensin altered the subsequent cyclic AMP-dependent phosphorylation of only 1 minor phosphoprotein substrate. 3. These results are consistent with other evidence which implicates calcium as an important intracellular mediator of the neurotensin signal.

制备大鼠脑尾状核切片，并用5微摩尔神经降压素孵育30秒和5分钟。在尾状核切片匀浆后，蛋白质在氯化钙或环磷酸腺苷存在的情况下进行体外磷酸化。磷酸化蛋白质通过电泳分离，通过所得放射自显影片的显微密度测定法定量单个蛋白质中的磷酸盐掺入量。2. 用神经降压素孵育尾状核切片改变了几种特定蛋白质底物随后的体外钙依赖性磷酸化，但相比之下，用神经降压素孵育仅改变了一种次要磷蛋白底物随后的环磷酸腺苷依赖性磷酸化。3. 这些结果与其他证据一致，这些证据表明钙是神经降压素信号的重要细胞内介质。

Cain S T, Nemeroff C B

Department of Psychiatry, Duke University Medical Center, Durham, NC.

Prog Neuropsychopharmacol Biol Psychiatry. 1991;15(1):83-9. doi: 10.1016/0278-5846(91)90043-z.

Caudate nucleus slices from rat brain were prepared and incubated with 5 microM neurotensin for 30 seconds and 5 minutes. Following homogenization of the caudate nucleus slices, proteins were phosphorylated in vitro in the presence of CaCl2 or cyclic AMP. Phosphorylated proteins were separated by electrophoresis, and phosphate incorporation into individual proteins quantitated by microdensitometry of the resultant autoradiographs. 2. Incubation of caudate nucleus slices with neurotensin altered the subsequent in vitro calcium-dependent phosphorylation of several specific protein substrates, but in contrast, incubation with neurotensin altered the subsequent cyclic AMP-dependent phosphorylation of only 1 minor phosphoprotein substrate. 3. These results are consistent with other evidence which implicates calcium as an important intracellular mediator of the neurotensin signal.

制备大鼠脑尾状核切片，并用5微摩尔神经降压素孵育30秒和5分钟。在尾状核切片匀浆后，蛋白质在氯化钙或环磷酸腺苷存在的情况下进行体外磷酸化。磷酸化蛋白质通过电泳分离，通过所得放射自显影片的显微密度测定法定量单个蛋白质中的磷酸盐掺入量。2. 用神经降压素孵育尾状核切片改变了几种特定蛋白质底物随后的体外钙依赖性磷酸化，但相比之下，用神经降压素孵育仅改变了一种次要磷蛋白底物随后的环磷酸腺苷依赖性磷酸化。3. 这些结果与其他证据一致，这些证据表明钙是神经降压素信号的重要细胞内介质。