多重串联聚合酶链反应可从单个福尔马林固定、石蜡包埋的乳腺癌切片中鉴定出雌激素受体和 Her-2 的强表达。

Multiplexed tandem polymerase chain reaction identifies strong expression of oestrogen receptor and Her-2 from single, formalin-fixed, paraffin-embedded breast cancer sections.

机构信息

University of Melbourne, Department of Surgery, Australia.

出版信息

Pathology. 2010 Feb;42(2):165-72. doi: 10.3109/00313020903494102.

DOI:10.3109/00313020903494102

PMID:20085519

Abstract

AIM

To establish the suitability of multiplex tandem polymerase chain reaction (MT-PCR) for rapid identification of oestrogen receptor (ER) and Her-2 status using a single, formalin-fixed, paraffin-embedded (FFPE) breast tumour section.

METHODS

Tissue sections from 29 breast tumours were analysed by immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH). RNA extracted from 10 mum FFPE breast tumour sections from 24 of 29 tumours (14 ER positive and 5 Her-2 positive) was analysed by MT-PCR. After establishing a correlation between IHC and/or FISH and MT-PCR results, the ER/Her-2 status of a further 32 randomly selected, archival breast tumour specimens was established by MT-PCR in a blinded fashion, and compared to IHC/FISH results.

RESULTS

MT-PCR levels of ER and Her-2 showed good concordance with IHC and FISH results. Furthermore, among the ER positive tumours, MT-PCR provided a quantitative score with a high dynamic range. Threshold values obtained from this data set applied to 32 archival tumour specimens showed that tumours strongly positive for ER and/or Her-2 expression were easily identified by MT-PCR.

CONCLUSION

MT-PCR can provide rapid, sensitive and cost-effective analysis of FFPE material and may prove useful as triage to identify patients suited to endocrine or trastuzumab (Herceptin) treatment.

摘要

目的

利用多重串联聚合酶链反应（MT-PCR），通过单一的福尔马林固定、石蜡包埋（FFPE）的乳腺肿瘤切片，快速鉴定雌激素受体（ER）和 Her-2 状态。

方法

对 29 例乳腺肿瘤的组织切片进行免疫组织化学（IHC）和荧光原位杂交（FISH）分析。从 29 例肿瘤中的 24 例（14 例 ER 阳性，5 例 Her-2 阳性）的 10 µm FFPE 乳腺肿瘤切片中提取 RNA，通过 MT-PCR 进行分析。在建立 IHC 和/或 FISH 与 MT-PCR 结果之间的相关性之后，以盲法方式通过 MT-PCR 对另外 32 例随机选择的存档乳腺肿瘤标本的 ER/Her-2 状态进行建立，并与 IHC/FISH 结果进行比较。