University of Melbourne, Department of Surgery, Australia.
Pathology. 2010 Feb;42(2):165-72. doi: 10.3109/00313020903494102.
To establish the suitability of multiplex tandem polymerase chain reaction (MT-PCR) for rapid identification of oestrogen receptor (ER) and Her-2 status using a single, formalin-fixed, paraffin-embedded (FFPE) breast tumour section.
Tissue sections from 29 breast tumours were analysed by immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH). RNA extracted from 10 mum FFPE breast tumour sections from 24 of 29 tumours (14 ER positive and 5 Her-2 positive) was analysed by MT-PCR. After establishing a correlation between IHC and/or FISH and MT-PCR results, the ER/Her-2 status of a further 32 randomly selected, archival breast tumour specimens was established by MT-PCR in a blinded fashion, and compared to IHC/FISH results.
MT-PCR levels of ER and Her-2 showed good concordance with IHC and FISH results. Furthermore, among the ER positive tumours, MT-PCR provided a quantitative score with a high dynamic range. Threshold values obtained from this data set applied to 32 archival tumour specimens showed that tumours strongly positive for ER and/or Her-2 expression were easily identified by MT-PCR.
MT-PCR can provide rapid, sensitive and cost-effective analysis of FFPE material and may prove useful as triage to identify patients suited to endocrine or trastuzumab (Herceptin) treatment.
利用多重串联聚合酶链反应（MT-PCR），通过单一的福尔马林固定、石蜡包埋（FFPE）的乳腺肿瘤切片，快速鉴定雌激素受体（ER）和 Her-2 状态。
对 29 例乳腺肿瘤的组织切片进行免疫组织化学（IHC）和荧光原位杂交（FISH）分析。从 29 例肿瘤中的 24 例（14 例 ER 阳性，5 例 Her-2 阳性）的 10 µm FFPE 乳腺肿瘤切片中提取 RNA，通过 MT-PCR 进行分析。在建立 IHC 和/或 FISH 与 MT-PCR 结果之间的相关性之后，以盲法方式通过 MT-PCR 对另外 32 例随机选择的存档乳腺肿瘤标本的 ER/Her-2 状态进行建立，并与 IHC/FISH 结果进行比较。
ER 和 Her-2 的 MT-PCR 水平与 IHC 和 FISH 结果具有良好的一致性。此外，在 ER 阳性肿瘤中，MT-PCR 提供了一个具有高动态范围的定量评分。从该数据集获得的阈值值应用于 32 例存档肿瘤标本表明，MT-PCR 可轻松识别 ER 和/或 Her-2 表达强阳性的肿瘤。
MT-PCR 可对 FFPE 材料进行快速、敏感且具有成本效益的分析，可能有助于作为分类鉴定，以确定适合内分泌或曲妥珠单抗（赫赛汀）治疗的患者。
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