EMBL-CRG Systems Biology Unit, Design of Biological Systems, Centre de Regulació Genòmica, Dr. Aiguader 88, 08003 Barcelona, Spain.
Cell. 2010 Jan 8;140(1):33-47. doi: 10.1016/j.cell.2009.12.028.
Cells respond to their environment by sensing signals and translating them into changes in gene expression. In recent years, synthetic networks have been designed in both prokaryotic and eukaryotic systems to create new functionalities and for specific applications. In this review, we discuss the challenges associated with engineering signal transduction pathways. Furthermore, we address advantages and disadvantages of engineering signaling pathways in prokaryotic and eukaryotic cells, highlighting recent examples, and discuss how progress in synthetic biology might impact biotechnology and biomedicine.
细胞通过感知信号并将其转化为基因表达的变化来对其环境做出反应。近年来，人们已经在原核和真核系统中设计了合成网络，以创造新的功能并应用于特定领域。在这篇综述中，我们讨论了工程信号转导途径所面临的挑战。此外，我们还讨论了在原核细胞和真核细胞中工程化信号通路的优缺点，重点介绍了最近的一些例子，并讨论了合成生物学的进展如何可能影响生物技术和生物医学。
