Department of Bionano Technology, Kyungwon University, Sungnam-si, Kyungki-do 461-701, South Korea.
Biochem Biophys Res Commun. 2010 Feb 19;392(4):505-9. doi: 10.1016/j.bbrc.2010.01.053. Epub 2010 Jan 18.
This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected hamsters while MDS revealed no PrPd in identically-treated plasma of healthy animals. This is the first report of a single ELISA- based immune detection of PrPd from blood samples.
本通讯描述了改良夹心酶联免疫吸附测定（ELISA）在检测仓鼠血液中与疾病相关的朊病毒蛋白（PrPd）多聚体形式中的应用，该方法称为多聚体检测系统（MDS）。MDS 可检测到朊病毒感染的仓鼠的血浆中的 PrPd，而在经过相同处理的健康动物的血浆中，MDS 则未检测到 PrPd。这是首个基于 ELISA 的从血液样本中免疫检测 PrPd 的报道。
