Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, D.F. 04510, Mexico.
Neurosci Lett. 2010 Mar 3;471(2):109-13. doi: 10.1016/j.neulet.2010.01.021. Epub 2010 Jan 18.
Recently, the effects of extremely low-frequency electromagnetic fields (ELF EMF) on biological systems have been extensively investigated. In this report, the influence of ELF EMF on olfactory bulb (OB) estrogen receptor-alpha (ER alpha) mRNA and -beta (ER beta) mRNA expression was studied by RT-PCR in adult female and male rats. Results reveal for the first time that ELF EMF exerted a biphasic effect on female OB ER beta mRNA gene expression, which increased during diestrous and decreased during estrous. We did not observe any influence of ELF EMF on female OB ER alpha mRNA expression. Our data demonstrate a fluctuating pattern of ER-alpha and -beta mRNA expression in the female OB throughout the phases of the estrous cycle in non-ELF EMF-exposed animals. Thus the highest ER alpha expression was observed in diestrous and the lowest in proestrous. The pattern of ER beta mRNA was less variable, the lowest expression was observed in diestrous. ER-alpha mRNA and -beta mRNA expression level in the male OB did not exhibit any variation either in ELF EMF-exposed or non-ELF EMF-exposed animals. In summary, ELF EMF modulate ER beta gene expression in the OB of female adult rats but not in males.
最近，人们广泛研究了极低频电磁场（ELF EMF）对生物系统的影响。在本报告中，我们通过 RT-PCR 研究了 ELF EMF 对成年雌性和雄性大鼠嗅球（OB）中雌激素受体-α（ER alpha）mRNA 和 -β（ER beta）mRNA 表达的影响。结果首次表明，ELF EMF 对雌性 OB ER beta mRNA 基因表达具有双相作用，即在发情间期增加，在发情期减少。我们没有观察到 ELF EMF 对雌性 OB ER alpha mRNA 表达的任何影响。我们的数据表明，在非 ELF EMF 暴露的动物中，雌性 OB 中的 ER-alpha 和 -beta mRNA 表达在发情周期的各个阶段呈现波动模式。因此，在发情间期观察到最高的 ER alpha 表达，在发情前期观察到最低的表达。ER beta mRNA 的表达模式变化较小，在发情间期观察到最低的表达。暴露于 ELF EMF 或非 ELF EMF 的雄性 OB 中的 ER-alpha mRNA 和 -beta mRNA 表达水平均无变化。总之，ELF EMF 调节雌性成年大鼠 OB 中的 ER beta 基因表达，但不调节雄性大鼠 OB 中的 ER beta 基因表达。
