Department of Periodontology, School of Dentistry, Kyungpook National University, Jung-Gu, Daegu, Republic of Korea.
Acta Biomater. 2010 Jul;6(7):2843-51. doi: 10.1016/j.actbio.2010.01.017. Epub 2010 Jan 18.
This study investigated the surface characteristics, in vitro and in vivo biocompatibility of Ti-6Al-4V alloy implants incorporating strontium ions (Sr), produced by hydrothermal treatment using a Sr-containing solution, for future biomedical applications. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, X-ray photoelectron spectroscopy, optical profilometry, contact angle and surface energy measurement and inductively coupled plasma-mass spectroscopy (ICP-MS). Human osteoblast-like cell (MG63) attachment, proliferation, alkaline phosphatase (ALP) activity, and quantitative analysis of osteoblastic gene expression on Sr-containing Ti-6Al-4V surfaces were compared with untreated Ti-6Al-4V surfaces. Fifty-six screw implants (28 control and 28 experimental) were placed in the tibiae and femoral condyles of seven New Zealand White rabbits. The osteoconductivity of Sr-containing Ti-6Al-4V implants was evaluated by removal torque testing and histomorphometric analysis after 4weeks implantation. Hydrothermal treatment produced a crystalline SrTiO(3) layer. ICP-MS analysis showed that Sr ions were released from treated surfaces into the solution. Significant increases in ALP activity (P=0.000), mRNA expressions of key osteoblast genes (osterix, bone sialoprotein, and osteocalcin), removal torque values (P<0.05) and bone-implant contact percentages (P<0.05) in both cortical and cancellous bone were observed for Sr-containing Ti-6Al-4V surfaces. The results indicate that the Sr-containing oxide layer produced by hydrothermal treatment may be effective in improving the osseointegration of Ti-6Al-4V alloy implants by enhancing differentiation of osteoblastic cells, removal torque forces and bone apposition in both cortical and cancellous bone.
本研究通过水热处理，使用含 Sr 溶液在 Ti-6Al-4V 合金植入物表面生成 Sr 离子，以探索表面特性、体外和体内生物相容性，为未来生物医学应用提供参考。采用扫描电子显微镜、薄膜 X 射线衍射仪、X 射线光电子能谱、光学轮廓仪、接触角和表面能测量以及电感耦合等离子体质谱（ICP-MS）等手段评估表面特性。将含 Sr 的 Ti-6Al-4V 表面与未处理的 Ti-6Al-4V 表面进行比较，评估人成骨样细胞（MG63）在含 Sr 表面的黏附、增殖、碱性磷酸酶（ALP）活性和成骨基因表达的定量分析。将 56 个螺钉植入物（28 个对照和 28 个实验）分别植入 7 只新西兰白兔的胫骨和股骨髁。4 周植入后，通过去除扭矩测试和组织形态计量学分析评估含 Sr 的 Ti-6Al-4V 植入物的骨传导性。水热处理生成了结晶 SrTiO(3)层。ICP-MS 分析表明，Sr 离子从处理表面释放到溶液中。在含 Sr 的 Ti-6Al-4V 表面观察到 ALP 活性（P=0.000）、关键成骨基因（osterix、骨涎蛋白和骨钙素）的 mRNA 表达、去除扭矩值（P<0.05）和皮质骨和松质骨中的骨-植入物接触百分比（P<0.05）均显著增加。结果表明，水热处理生成的含 Sr 氧化物层可能通过增强成骨细胞分化、增加皮质骨和松质骨中的去除扭矩力和骨附着，从而提高 Ti-6Al-4V 合金植入物的骨整合。
