Hu Guo, Wang Shou-Zhi, Zhang Sen, Chen Wei-Xing, Liu Shuang, Tian Jian-Wei, Li Hui
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Yi Chuan. 2010 Jan;32(1):59-66. doi: 10.3724/sp.j.1005.2010.00059.
It has been found that epistasis for selective response plays an indispensible role in animal genetics and breeding. In this study, the polymorphisms of T123G in apoliprotein B (ApoB) and C1197A in uncoupling protein (UCP) among individuals from the 8th to the 10th generation populations of the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHFL) were detected, and genetic analysis of the epistatic effects between the two SNPs on abdominal fat percentage (AFP) was performed using Natural and Orthogonal InterActions (NOIA) model. According to these assays, we concluded that at least one out of four epistatic components between these two SNPs was significantly associated with AFP (Plt;0.05) in fat lines from the 8th to the 10th generations of NEAUHFL; on the contrary, none was significantly associated with AFP (P>0.05) in lean lines. Our results suggested that epistatic interactions among QTLs and functional SNPs in candidate genes affecting fat traits might lead to differences in growth patterns of fat traits between lean and fat chicken lines.
现已发现，上位性对选择反应在动物遗传育种中起着不可或缺的作用。本研究检测了东北农业大学高、低脂肉鸡第8至10世代群体个体中载脂蛋白B（ApoB）基因T123G和解偶联蛋白（UCP）基因C1197A的多态性，并利用自然和正交互作（NOIA）模型对这两个单核苷酸多态性（SNP）之间对腹脂率（AFP）的上位效应进行了遗传分析。根据这些测定，我们得出结论，在东北农业大学高、低脂肉鸡第8至10世代的脂肪系中，这两个SNP之间的四个上位性成分中至少有一个与AFP显著相关（P<0.05）；相反，在瘦肉系中，没有一个与AFP显著相关（P>0.05）。我们的结果表明，影响脂肪性状的候选基因中数量性状位点（QTL）和功能性SNP之间的上位性互作可能导致瘦肉型和脂肪型鸡品系之间脂肪性状生长模式的差异。
