Wu Xianwen, Zhang Qi, Xu Songsong, Jin Pengcheng, Luan Peng, Li Yumao, Cao Zhiping, Leng Li, Wang Yuxiang, Wang Shouzhi
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Mol Cell Probes. 2016 Feb;30(1):1-5. doi: 10.1016/j.mcp.2015.12.003. Epub 2015 Dec 30.
A genome-wide association study has shown a number of chicken (Gallus gallus) single nucleotide polymorphism (SNP) markers to be significantly associated with abdominal fat content in Northeast Agricultural University (NEAU) broiler lines selected divergently for abdominal fat content (NEAUHLF). The six significant SNPs are located in the kinase insert domain receptor (KDR), tumor suppressor candidate 3 (TUSC3), phosphoribosyl pyrophosphate amidotransferase (PPAT), exocyst complex component 1 (EXOC1), v-myb myeloblastosis viral oncogene homolog (avian)-like 2 (MYBL2) and KIAA1211 (undefined) genes. In this study, the expression levels of these genes were investigated in both abdominal fat and liver tissues using 32 14th generation chickens from the NEAUHLF. The levels of expression of KDR in abdominal fat and KDR and TUSC3 in liver differed significantly between the two lines. The expression level of KDR in the abdominal fat was significantly correlated with the abdominal fat weight (AFW) and abdominal fat percentage (AFP). The expression levels of KDR, TUSC3 and PPAT in liver were significantly correlated with AFW and AFP, indicating that the six genes, especially KDR and TUSC3, could be associated with fat traits in domestic chickens. This study could provide insight into the mechanisms underlying the formation of abdominal fat in chickens.
一项全基因组关联研究表明，在东北农业大学（NEAU）针对腹脂含量进行了不同方向选育的肉鸡品系（NEAUHLF）中，一些鸡（原鸡）单核苷酸多态性（SNP）标记与腹脂含量显著相关。这六个显著的SNP位于激酶插入结构域受体（KDR）、肿瘤抑制候选基因3（TUSC3）、磷酸核糖焦磷酸酰胺转移酶（PPAT）、外囊复合体成分1（EXOC1）、v-myb髓细胞白血病病毒癌基因同源物（禽类）样2（MYBL2）和KIAA1211（功能未明）基因中。在本研究中，使用来自NEAUHLF的32只第14代鸡，对这些基因在腹脂和肝脏组织中的表达水平进行了研究。两品系之间，腹脂中KDR的表达水平以及肝脏中KDR和TUSC3的表达水平存在显著差异。腹脂中KDR的表达水平与腹脂重量（AFW）和腹脂百分比（AFP）显著相关。肝脏中KDR、TUSC3和PPAT的表达水平与AFW和AFP显著相关，这表明这六个基因，尤其是KDR和TUSC3，可能与家鸡的脂肪性状有关。本研究可为鸡腹脂形成的潜在机制提供见解。
