RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Japan.
Mol Plant. 2010 Jan;3(1):125-42. doi: 10.1093/mp/ssp069. Epub 2009 Aug 26.
Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length (FL) cDNAs (rice FOX Arabidopsis lines) using a gas chromatography-time-of-flight mass spectrometry (GC-TOF/MS)-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candidate lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Arabidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries (LOB) Domain (LBD)/Asymmetric Leaves2-like (ASL) gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.
植物代谢组学作为一种强大的工具，可用于研究基因功能，并深入了解植物细胞的生理学。在这项研究中，我们使用基于气相色谱-飞行时间质谱（GC-TOF/MS）的技术筛选过量表达水稻全长（FL）cDNA 的拟南芥系（水稻 FOX 拟南芥系），以鉴定导致代谢变化的水稻基因。该筛选系统允许快速可靠地鉴定显示代谢物图谱改变的候选系。我们对一个水稻 FOX 拟南芥系进行了代谢组学和转录组学分析，该系含有水稻侧生器官边界（LOB）域（LBD）/不对称叶 2 样（ASL）基因的水稻直系同源物（LOC_Os11g44610）的 FL cDNA。研究的水稻 FOX 拟南芥系显示出与氮代谢相关的代谢物水平的显著变化。转录组数据以及拟南芥 At-LBD37/ASL39 过表达植物的代谢物分析结果与这些发现一致。此外，对 Os-LBD37/ASL39 过表达水稻植物的代谢组学和转录组学分析表明，Os-LBD37/ASL39 与水稻氮代谢相关过程有关。因此，基于代谢组学的筛选方法和功能获得方法的结合，可用于快速鉴定拟南芥和水稻中的新基因。
