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放射性免疫沉淀法与荧光素酶免疫沉淀法检测谷氨酸脱羧酶 65 自身抗体和胰岛细胞自身抗体的比较。

Comparison of radioimmunoprecipitation with luciferase immunoprecipitation for autoantibodies to GAD65 and IA-2beta.

机构信息

Neurobiology and Pain Therapeutics Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.

出版信息

Diabetes Care. 2010 Apr;33(4):754-6. doi: 10.2337/dc09-1938. Epub 2010 Jan 19.

DOI:10.2337/dc09-1938
PMID:20086252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2845022/
Abstract

OBJECTIVE

To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2beta.

RESEARCH DESIGN AND METHODS

Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2beta.

RESULTS

LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2beta by LIPS and RIP were not statistically different.

CONCLUSIONS

The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

摘要

目的

比较荧光素酶免疫沉淀（LIPS）与放射免疫沉淀（RIP）检测 1 型糖尿病自身抗原谷氨酸脱羧酶 65（GAD65）和胰岛细胞相关蛋白 2β（IA-2β）自身抗体的灵敏度和特异性。

研究设计和方法

使用 2007 年糖尿病抗体标准化计划的 49 例 1 型糖尿病患者和 100 名非糖尿病对照者的血清来筛选 GAD65 自身抗体。另外 200 例 1 型糖尿病患者和 200 名非糖尿病对照者用于验证 GAD65 结果并筛选 IA-2β 自身抗体。

结果

LIPS 检测 GAD65 和 IA-2β 自身抗体的灵敏度和特异性与 RIP 检测相同。受试者工作特征分析显示，LIPS 和 RIP 检测 GAD65 和 IA-2β 自身抗体的检测结果无统计学差异。

结论

LIPS 检测法不需要使用放射性同位素或体外转录/翻译，是 RIP 检测法在临床水平上的一种实用替代方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ff9/2845022/53bbd13c2147/zdc0041081770001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ff9/2845022/53bbd13c2147/zdc0041081770001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ff9/2845022/53bbd13c2147/zdc0041081770001.jpg

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