质子离子液体中的可溶性、折叠和活性枯草杆菌蛋白酶。
Soluble, folded and active subtilisin in a protic ionic liquid.
机构信息
CNAP, Department of Biology, University of York, York, United Kingdom YO10 5YW.
出版信息
Chem Commun (Camb). 2010 Feb 7;46(5):749-51. doi: 10.1039/b916497a. Epub 2009 Nov 26.
DOI:10.1039/b916497a
PMID:20087508
Abstract
The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.
摘要
研究了糜蛋白酶和枯草杆菌蛋白酶在一系列质子羟烷基铵离子液体中的活性，并用 APEE（N-乙酰-L-苯丙氨酸乙酯）作为模型底物进行了测试；仅在二乙醇铵氯化物（DEA Cl）中观察到枯草杆菌蛋白酶的活性，而在测试的任何 PIL 中，糜蛋白酶均没有活性。
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