Department of Surgery, Taipei Medical University, Taipei, Taiwan.
Ann Surg Oncol. 2010 Jun;17(6):1703-9. doi: 10.1245/s10434-010-0912-8. Epub 2010 Jan 20.
GRP78 plays an essential role in embryonic development and in the therapeutic treatment and progression of cancer. However, little is known about the role of GRP78 in hepatocellular carcinoma (HCC).
In this study, we characterized five different HCC cell lines to examine GRP78 expression patterns and found that only HepJ5 cells ectopically overexpress GRP78. We knocked down GRP78 expression in HepJ5 cells using a small interfering RNA (siRNA), and the proliferation assay and migration assay were performed.
Using siRNA technique, we could successfully reduce GRP78 expression levels in HepJ5 cells. In a cell growth study, we found that GRP78-siRNA caused no significant changes in cellular proliferation in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, and cell cycle distribution. In a cell migration study, we found that GRP78-siRNA HepJ5 cells had dramatically increased migration ability in Transwell assay.
We conclude that ectopically expressed GRP78 does not contribute to the increased proliferation of HepJ5 cells, but does correlate with the migration of HCC cells under normoxic conditions.
GRP78 在胚胎发育以及癌症的治疗和进展中起着至关重要的作用。然而，关于 GRP78 在肝细胞癌（HCC）中的作用知之甚少。
在本研究中，我们对五种不同的 HCC 细胞系进行了特征描述，以检查 GRP78 的表达模式，发现只有 HepJ5 细胞过表达 GRP78。我们使用小干扰 RNA（siRNA）敲低 HepJ5 细胞中的 GRP78 表达，并进行了增殖测定和迁移测定。
使用 siRNA 技术，我们可以成功降低 HepJ5 细胞中的 GRP78 表达水平。在细胞生长研究中，我们发现 GRP78-siRNA 对 MTT 细胞增殖、集落形成和细胞周期分布没有显著影响。在细胞迁移研究中，我们发现 GRP78-siRNA HepJ5 细胞在 Transwell 测定中迁移能力显著增强。
我们得出结论，过表达的 GRP78 不会促进 HepJ5 细胞的增殖，但与 HCC 细胞在常氧条件下的迁移相关。
