Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.
Ann Surg Oncol. 2010 Jun;17(6):1695-702. doi: 10.1245/s10434-010-0911-9. Epub 2010 Jan 20.
Gastric carcinoma is one of the most common malignancies in the world, yet little is known about the molecular process of its development and progression. The aims of this study are to correlate the expression of nuclear protein kinase CK2 beta subunit (CK2beta) with clinicopathologic parameters and patient survival.
Expression levels of nuclear CK2beta were analyzed in 104 gastric tissues from patients with gastric carcinoma by immunohistochemistry. A paired t test was used to analyze the differences in nuclear CK2beta expression between tumor and nontumor tissues in the same patient. A two-tailed chi (2) test was performed to determine the significance of the difference between nuclear CK2beta expression and clinicopathologic parameters. All time-to-event endpoints according to various clinicopathologic parameters were plotted by Kaplan-Meier method, and significance was then determined by univariate log-rank test. Cox proportional-hazards model was used for multivariate analysis to determine the independence of prognostic impact of nuclear CK2beta expression.
Overexpression of nuclear CK2beta was significantly correlated with depth of invasion (P = 0.042). Patients with high expression levels of nuclear CK2beta had a significantly poorer overall survival rate compared with patients with low expression levels of nuclear CK2beta (P = 0.0006). On multivariate Cox regression analysis, overexpression of nuclear CK2beta and stage were proven to be independent prognostic markers for gastric carcinoma (P = 0.0036 and 0.0005, respectively).
Overexpression of nuclear CK2beta can be a useful marker for predicting the outcome of patients with gastric carcinoma.
胃癌是世界上最常见的恶性肿瘤之一，但对于其发展和进展的分子过程知之甚少。本研究旨在探讨核蛋白激酶 CK2β亚单位（CK2β）的表达与临床病理参数和患者生存的关系。
采用免疫组织化学法检测 104 例胃癌组织中核 CK2β的表达水平。采用配对 t 检验分析同一患者肿瘤组织和非肿瘤组织核 CK2β表达的差异。采用双侧 χ²检验分析核 CK2β表达与临床病理参数的差异。根据各种临床病理参数绘制所有时间事件终点的 Kaplan-Meier 曲线，并用单因素对数秩检验确定差异的显著性。采用 Cox 比例风险模型进行多因素分析，确定核 CK2β表达对预后的独立影响。
核 CK2β的过表达与浸润深度显著相关（P=0.042）。核 CK2β高表达组患者的总生存率明显低于核 CK2β低表达组（P=0.0006）。多因素 Cox 回归分析显示，核 CK2β过表达和肿瘤分期是胃癌的独立预后标志物（P=0.0036 和 0.0005）。
核 CK2β的过表达可以作为预测胃癌患者预后的有用标志物。
