Lin Kai-Yuan, Fang Chia-Lang, Uen Yih-Huei, Chang Chun-Chao, Lou Horng-Yuan, Hsieh Ching-Ruey, Tiong Cheng, Pan Shiann, Chen Sheng-Hsuan
Division of Gastroenterology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan.
J Surg Oncol. 2008 May 1;97(6):538-43. doi: 10.1002/jso.20997.
The variability of the prognosis of gastric carcinoma drives extensive researches for novel prognostic markers. The aims of this study were to correlate the expression of protein kinase Calpha (PKCalpha) mRNA with clinicopathological parameters and to evaluate the significant value of PKCalpha in gastric carcinoma prognosis.
PKCalpha mRNA levels were analyzed in tumor/non-tumor pairs of gastric tissues from surgical specimens of 41 patients with gastric carcinoma employing quantitative real-time polymerase chain reaction. Expression of PKCalpha in gastric carcinoma was also examined using immunohistochemistry.
PKCalpha mRNA expression was significantly upregulated in gastric carcinoma (P = 0.007). Overexpression of PKCalpha mRNA was correlated with distant metastasis (P = 0.040). Patients with high PKCalpha mRNA expression had a significantly poorer overall survival compared with patients with low PKCalpha mRNA expression (P = 0.0113). The uni-variate Cox regression analysis showed that high PKCalpha mRNA expression (P = 0.0363) and depth of invasion (P = 0.0443) were two significant prognostic markers for gastric carcinoma. In backward stepwise multi-variate analysis, PKCalpha mRNA overexpression was also proved to be an independent prognostic marker for gastric carcinoma (P = 0.0275).
Our results suggest that overexpression of PKCalpha mRNA has correlation with distant metastasis and may be an independent prognostic marker for gastric carcinoma.
胃癌预后的变异性推动了对新型预后标志物的广泛研究。本研究的目的是将蛋白激酶Cα（PKCα）mRNA的表达与临床病理参数相关联，并评估PKCα在胃癌预后中的重要价值。
采用定量实时聚合酶链反应分析41例胃癌手术标本的肿瘤/非肿瘤胃组织对中PKCα mRNA水平。还使用免疫组织化学检测胃癌中PKCα的表达。
胃癌中PKCα mRNA表达显著上调（P = 0.007）。PKCα mRNA的过表达与远处转移相关（P = 0.040）。与低PKCα mRNA表达的患者相比，高PKCα mRNA表达的患者总生存期明显较差（P = 0.0113）。单变量Cox回归分析表明，高PKCα mRNA表达（P = 0.0363）和浸润深度（P = 0.0443）是胃癌的两个重要预后标志物。在向后逐步多变量分析中，PKCα mRNA过表达也被证明是胃癌的独立预后标志物（P = 0.0275）。
我们的结果表明，PKCα mRNA的过表达与远处转移相关，可能是胃癌的独立预后标志物。
